ArticleNucleic acids research2008
Efficient transfer of base changes from a vector to the rice genome by homologous recombination: involvement of heteroduplex formation and mismatch correction.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Molecular identification and studies on genetic diversity and structure-related GC heterogeneity of Spatholobus Suberectus based on ITS2.Scientific reports · 2024Article
- A rapid aureochrome opto-switch enables diatom acclimation to dynamic light.Nature communications · 2024Article
- Targeted deletion of grape retrotransposon associated with fruit skin color via CRISPR/Cas9 in Vitis labrascana 'Shine Muscat'.PloS one · 2023Article
- Precision genome editing in plants via gene targeting and subsequent break-induced single-strand annealing.Plant biotechnology journal · 2021Article
- Rice Gene Targeting by Homologous Recombination with Positive-Negative Selection Strategy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants.Frontiers in genome editing · 2020Article
- Biallelic Gene Targeting in Rice.Plant physiology · 2016Article
- Precision genome editing in plants via gene targeting and piggyBac-mediated marker excision.The Plant journal : for cell and molecular biology · 2015Article
- The MET1b gene encoding a maintenance DNA methyltransferase is indispensable for normal development in rice.Plant molecular biology · 2014Article
- Positive-negative-selection-mediated gene targeting in rice.Frontiers in plant science · 2014Review
- Poplars with a PtDDM1-RNAi transgene have reduced DNA methylation and show aberrant post-dormancy morphology.Planta · 2013Article
- Application of gene targeting to designed mutation breeding of high-tryptophan rice.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Gene targeting refers to the alteration of a specific DNA sequence in an endogenous gene at its original locus in the genome by homologous recombination. Through a gene-targeting procedure with positive-negative selection, we previously reported the generation of fertile transgenic rice plants with a positive marker inserted into the Adh2 gene by using an Agrobacterium-mediated transformation vector containing the positive marker flanked by two 6-kb homologous segments for recombination. We describe here that base changes within the homologous segments in the vector could be efficiently transferred into the corresponding genomic sequences of rice recombinants. Interestingly, a few sequences from the host genome were flanked by the changed sequences derived from the vector in most of the recombinants. Because a single-stranded T-DNA molecule in Agrobacterium-mediated transformation is imported into the plant nucleus and becomes double-stranded, both single-stranded and double-stranded T-DNA intermediates can serve in gene-targeting processes. Several alternative models, including the occurrence of the mismatch correction of heteroduplex molecules formed between the genomic DNA and either a single-stranded or double-stranded T-DNA intermediate, are compared to explain the observation, and implications for the modification of endogenous genes for functional genomic analysis by gene targeting are discussed.
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