ArticleProceedings of the National Academy of Sciences of the United States of America2008
Two isozymes of particulate methane monooxygenase with different methane oxidation kinetics are found in Methylocystis sp. strain SC2.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers, 1 of them a synthesis that pooled it.
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93 citing papers in PubMed, 1 synthesis or guideline pooled it, 279 citations in OpenAlex.
- Leveraging genome-scale metabolic models to understand aerobic methanotrophs.The ISME journal · 2024Pooled it
- Evading native restriction-modification systems improves electroporation efficiency of the methanotrophApplied and environmental microbiology · 2026Article
- Environmental Preferences and Functional Variations of Methanotrophs in Northeast Qinghai-Tibet Plateau Wetlands.Environmental microbiology reports · 2026Article
- Unraveling underlying mechanisms and responsible microbes of organic residue-stimulated atmospheric methane uptake in agricultural soils.FEMS microbiology ecology · 2026Article
- Ammonia monooxygenase: a work in progress.Chemical science · 2026Review
- Microbial interaction networks as climate thermometers: redefining temperature sensitivity of aerobic methanotrophy in freshwater ecosystems.npj biodiversity · 2026Article
- High-efficiency methane consumption by atmospheric methanotrophs in subsurface karst caves: The irrefutable methane sink.Science advances · 2026Article
- Uncovering hidden phylo- and ecogenomic diversity of the widespread methanotrophic genus Methylobacter.FEMS microbiology ecology · 2026Article
- Methanotrophic microbiomes for dilute coal-mine methane biofiltration: ecophysiological constraints and design priorities.Frontiers in microbiology · 2026Review
- Article
- Investigation on the aerobic methanotrophic community and the dominant taxon Methylomarinum in seagrass ecosystem.Environmental microbiome · 2025Article
- Effects of drainage and long-term tillage on greenhouse gas fluxes in a natural wetland: insights from microbial mechanisms.Environmental microbiome · 2025Article
- Methane-cycling microbiomes in soils of the pan-Arctic and their response to permafrost degradation.Communications earth & environment · 2025Article
- Methylocystis borbori sp.nov., a novel methanotrophic bacterium from the sludge of a freshwater lake and its metabolic properties.Antonie van Leeuwenhoek · 2024Article
- Isolation of a facultative methanotroph Methylocystis iwaonis SD4 from rice rhizosphere and establishment of rapid genetic tools for it.Biotechnology letters · 2024Article
- Identifying Active Rather than Total Methanotrophs Inhabiting Surface Soil Is Essential for the Microbial Prospection of Gas Reservoirs.Microorganisms · 2024Article
- Role of methanotrophic communities in atmospheric methane oxidation in paddy soils.Frontiers in microbiology · 2024Article
- A methanotrophic bacterium to enable methane removal for climate mitigation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Acidophilic methanotrophs: Occurrence, diversity, and possible bioremediation applications.Environmental microbiology reports · 2023Review
- Asparagine Uptake: a Cellular Strategy ofApplied and environmental microbiology · 2023Article
33 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
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Abstract
Methane-oxidizing bacteria (methanotrophs) attenuate methane emission from major sources, such as wetlands, rice paddies, and landfills, and constitute the only biological sink for atmospheric methane in upland soils. Their key enzyme is particulate methane monooxygenase (pMMO), which converts methane to methanol. It has long been believed that methane at the trace atmospheric mixing ratio of 1.75 parts per million by volume (ppmv) is not oxidized by the methanotrophs cultured to date, but rather only by some uncultured methanotrophs, and that type I and type II methanotrophs contain a single type of pMMO. Here, we show that the type II methanotroph Methylocystis sp. strain SC2 possesses two pMMO isozymes with different methane oxidation kinetics. The pmoCAB1 genes encoding the known type of pMMO (pMMO1) are expressed and pMMO1 oxidizes methane only at mixing ratios >600 ppmv. The pmoCAB2 genes encoding pMMO2, in contrast, are constitutively expressed, and pMMO2 oxidizes methane at lower mixing ratios, even at the trace level of atmospheric methane. Wild-type strain SC2 and mutants expressing pmoCAB2 but defective in pmoCAB1 consumed atmospheric methane for >3 months. Growth occurred at 10-100 ppmv methane. Most type II but no type I methanotrophs possess the pmoCAB2 genes. The apparent K(m) of pMMO2 (0.11 muM) in strain SC2 corresponds well with the K(m(app)) values for methane oxidation measured in soils that consume atmospheric methane, thereby explaining why these soils are dominated by type II methanotrophs, and some by Methylocystis spp., in particular. These findings change our concept of methanotroph ecology.
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