ArticleRetrovirology2008
CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45: no evidence for a distinct exosome/HIV-1 budding pathway.
Article in Retrovirology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 60 citations in OpenAlex.
- Mucosal-Associated Invariant T cells exhibit distinct functional signatures associated with protection against typhoid fever.Cellular immunology · 2022Article
- Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques.Biosensors · 2021Review
- Recent Advancement and Technical Challenges in Developing Small Extracellular Vesicles for Cancer Drug Delivery.Pharmaceutical research · 2021Review
- A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- B Cells Control Mucosal-Associated Invariant T Cell Responses toFrontiers in immunology · 2021Article
- Tumor-Derived Exosomal Protein Tyrosine Phosphatase Receptor Type O Polarizes Macrophage to Suppress Breast Tumor Cell Invasion and Migration.Frontiers in cell and developmental biology · 2021Article
- Extracellular Vesicles in Viral Replication and Pathogenesis and Their Potential Role in Therapeutic Intervention.Viruses · 2020Review
- Review
- Differential functional patterns of memory CD4Vaccine · 2020Article
- Fat Therapeutics: The Clinical Capacity of Adipose-Derived Stem Cells and Exosomes for Human Disease and Tissue Regeneration.Frontiers in pharmacology · 2020Review
- The role of exosomal transport of viral agents in persistent HIV pathogenesis.Retrovirology · 2018Review
- Aqueous two-phase system to isolate extracellular vesicles from urine for prostate cancer diagnosis.PloS one · 2018Article
- Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells.PLoS neglected tropical diseases · 2017Article
- Polymorphic Nature of Human T-Cell Leukemia Virus Type 1 Particle Cores as Revealed through Characterization of a Chronically Infected Cell Line.Journal of virology · 2017Article
- Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.Cell host & microbe · 2016Article
- Extracellular vesicle isolation and characterization: toward clinical application.The Journal of clinical investigation · 2016Review
- A novel multiplex bead-based platform highlights the diversity of extracellular vesicles.Journal of extracellular vesicles · 2016Article
- Article
- High-yield isolation of extracellular vesicles using aqueous two-phase system.Scientific reports · 2015Article
- Microfluidic isolation of cancer-cell-derived microvesicles from hetergeneous extracellular shed vesicle populations.Biomedical microdevices · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The presence of relatively high levels of cellular protein contamination in density-purified virion preparations is a confounding factor in biochemical analyses of HIV and SIV produced from hematopoietic cells. A major source of this contamination is from vesicles, either microvesicles or exosomes, that have similar physical properties as virions. Thus, these particles can not be removed by size or density fractionation. Although virions and vesicles have similar cellular protein compositions, CD45 is excluded from HIV-1 yet is present in vesicles produced from hematopoietic cells. By exploiting this finding, we have developed a CD45 immunoaffinity depletion procedure that removes vesicles from HIV-1 preparations. While this approach has been successfully applied to virion preparations from several different cell types, some groups have concluded that "exosomes" from certain T cell lines, specifically Jurkat, do not contain CD45. If this interpretation is correct, then these vesicles could not be removed by CD45 immunoaffinity depletion. Here we show that dense vesicles produced by Jurkat and SupT1/CCR5 cells contain CD45 and are efficiently removed from preparations by CD45-immunoaffinity depletion. Also, contaminating cellular proteins were removed from virion preparations produced by these lines. Previously, the absence of CD45 from both "exosomes" and virions has been used to support the so called Trojan exosome hypothesis, namely that HIV-1 is simply an exosome containing viral material. The presence of CD45 on vesicles, including exosomes, and its absence on virions argues against a specialized budding pathway that is shared by both exosomes and HIV-1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.