ArticleGenome biology2008
The transcriptional program underlying the physiology of clostridial sporulation.
Article in Genome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 187 citations in OpenAlex.
- Comparative Metabolomics ofJournal of microbiology and biotechnology · 2025Article
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- Single and multiplexed gene repression in solventogenicSynthetic and systems biotechnology · 2023Article
- Production of propionate using metabolically engineered strains of Clostridium saccharoperbutylacetonicum.Applied microbiology and biotechnology · 2022Article
- Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum.Nature communications · 2022Article
- Conservation and Evolution of the Sporulation Gene Set in Diverse Members of theJournal of bacteriology · 2022Article
- Occurrence of genes encoding spore germination inMicrobial genomics · 2022Article
- Comparative Transcriptome Analysis ofFrontiers in cellular and infection microbiology · 2022Article
- Crystal Structure and Molecular Mechanism of Phosphotransbutyrylase fromJournal of microbiology and biotechnology · 2021Article
- Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum.Microbial cell factories · 2021Article
- High Performance of Biohydrogen Production in Packed-Filter Bioreactor via Optimizing Packed-Filter Position.International journal of environmental research and public health · 2021Article
- Sporulation in solventogenic and acetogenic clostridia.Applied microbiology and biotechnology · 2021Review
- Biofilm and Spore Formation ofAntibiotics (Basel, Switzerland) · 2021Article
- Distribution and preservation of the components of the engulfment. What is beyond representative genomes?PloS one · 2021Article
- Article
- Bacterial Spore mRNA - What's Up With That?Frontiers in microbiology · 2020Review
- Gene coexpression network analysis reveals a novel metabolic mechanism ofBiotechnology for biofuels · 2020Article
- Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level.Scientific reports · 2019Article
- Diversity of bacteria associated with corn roots inoculated with Canadian woodland soils, and description ofHeliyon · 2018Article
- Small and Low but Potent: the Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium acetobutylicum.Applied and environmental microbiology · 2018Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundClostridia are ancient soil organisms of major importance to human and animal health and physiology, cellulose degradation, and the production of biofuels from renewable resources. Elucidation of their sporulation program is critical for understanding important clostridial programs pertaining to their physiology and their industrial or environmental applications.
resultsUsing a sensitive DNA-microarray platform and 25 sampling timepoints, we reveal the genome-scale transcriptional basis of the Clostridium acetobutylicum sporulation program carried deep into stationary phase. A significant fraction of the genes displayed temporal expression in six distinct clusters of expression, which were analyzed with assistance from ontological classifications in order to illuminate all known physiological observations and differentiation stages of this industrial organism. The dynamic orchestration of all known sporulation sigma factors was investigated, whereby in addition to their transcriptional profiles, both in terms of intensity and differential expression, their activity was assessed by the average transcriptional patterns of putative canonical genes of their regulon. All sigma factors of unknown function were investigated by combining transcriptional data with predicted promoter binding motifs and antisense-RNA downregulation to provide a preliminary assessment of their roles in sporulation. Downregulation of two of these sigma factors, CAC1766 and CAP0167, affected the developmental process of sporulation and are apparently novel sporulation-related sigma factors.
conclusionThis is the first detailed roadmap of clostridial sporulation, the most detailed transcriptional study ever reported for a strict anaerobe and endospore former, and the first reported holistic effort to illuminate cellular physiology and differentiation of a lesser known organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.