ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2008
Generation of flow cytometry data files with a potentially infinite number of dimensions.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Review
- A backbone-based flow cytometry approach to decipher regulatory T cell trajectories in the human thymus.Frontiers in immunology · 2025Article
- Flow cytometric analysis of myelodysplasia: Pre-analytical and technical issues-Recommendations from the European LeukemiaNet.Cytometry. Part B, Clinical cytometry · 2023Article
- CytofIn enables integrated analysis of public mass cytometry datasets using generalized anchors.Nature communications · 2022Article
- UMAP Based Anomaly Detection for Minimal Residual Disease Quantification within Acute Myeloid Leukemia.Cancers · 2022Article
- Expert-independent classification of mature B-cell neoplasms using standardized flow cytometry: a multicentric study.Blood advances · 2022Article
- Article
- Knowledge transfer to enhance the performance of deep learning models for automated classification of B cell neoplasms.Patterns (New York, N.Y.) · 2021Article
- Article
- High-throughput single-cell quantification of hundreds of proteins using conventional flow cytometry and machine learning.Science advances · 2021Article
- High-Dimensional Analysis of Single-Cell Flow Cytometry Data Predicts Relapse in Childhood Acute Lymphoblastic Leukaemia.Cancers · 2020Article
- Article
- Efficiency and Health Economic Evaluations of BD OneFlow™ Flow Cytometry Reagents for Diagnosing Chronic Lymphoid Leukemia.Cytometry. Part B, Clinical cytometry · 2019Article
- CyTOFmerge: integrating mass cytometry data across multiple panels.Bioinformatics (Oxford, England) · 2019Article
- CytoBackBone: an algorithm for merging of phenotypic information from different cytometric profiles.Bioinformatics (Oxford, England) · 2019Article
- Characterization of Leukocytes From HIV-ART Patients Using Combined Cytometric Profiles of 72 Cell Markers.Frontiers in immunology · 2019Article
- Minimal/Measurable Residual Disease Detection in Acute Leukemias by Multiparameter Flow Cytometry.Current hematologic malignancy reports · 2018Review
- Next Generation Flow for highly sensitive and standardized detection of minimal residual disease in multiple myeloma.Leukemia · 2017Article
- Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia.Blood · 2017Article
- Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality.Oncotarget · 2015Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Immunophenotypic characterization of B-cell chronic lymphoproliferative disorders (B-CLPD) is associated with the use of increasingly larger panels of multiple combinations of 3 to > or =6 monoclonal antibodies (Mab), data analysis being separately performed for each of the different stained sample aliquots. Here, we describe and validate an automated method for calculation of flow cytometric data from several multicolor stainings of the same cell sample--i.e., the merging of data from different aliquots stained with partially overlapping combinations of Mab reagents (focusing on > or =1 cell populations)--into one data file as if it concerned a single "super" multicolor staining. Evaluation of the performance of the method described was done in a group of 60 B-CLPD studied at diagnosis with 18 different reagents in a panel containing six different 3- and 4-color stainings, which systematically contained CD19 for the identification of B-cells. Our results show a high degree of correlation and agreement between originally measured and calculated data about cell surface stainings, providing a basis for the use of this approach for the generation of flow cytometric data files containing information about a virtually infinite number of stainings for each individual cellular event measured in a sample, using a limited number of fluorochrome stainings.
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