ArticleCellular and molecular neurobiology2008
Tissue plasminogen activator enhances the hypoxia/reoxygenation-induced impairment of the blood-brain barrier in a primary culture of rat brain endothelial cells.
Article in Cellular and molecular neurobiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- The dynamics of thrombolysis over time in acute immunologic reactions.Scientific reports · 2025Article
- An easy-to-perform method for microvessel isolation and primary brain endothelial cell culture to study Alzheimer's disease.Heliyon · 2024Article
- Thromboelastography in acute immunologic reactions: a prospective pilot study.Research and practice in thrombosis and haemostasis · 2024Article
- MAP Kinase Pathways in Brain Endothelial Cells and Crosstalk with Pericytes and Astrocytes Mediate Contrast-Induced Blood-Brain Barrier Disruption.Pharmaceutics · 2021Article
- The role of endogenous tissue-type plasminogen activator in neuronal survival after ischemic stroke: friend or foe?Cellular and molecular life sciences : CMLS · 2019Review
- The Effect of Therapeutic Mild Hypothermia on Brain Microvascular Endothelial Cells During Ischemia-Reperfusion Injury.Neurocritical care · 2018Article
- Regulatory T cells ameliorate tissue plasminogen activator-induced brain haemorrhage after stroke.Brain : a journal of neurology · 2017Article
- Stroke, Vascular Dementia, and Alzheimer's Disease: Molecular Links.Journal of Alzheimer's disease : JAD · 2016Review
- Progesterone protects endothelial cells after cerebrovascular occlusion by decreasing MCP-1- and CXCL1-mediated macrophage infiltration.Experimental neurology · 2015Article
- Candesartan improves ischemia-induced impairment of the blood-brain barrier in vitro.Cellular and molecular neurobiology · 2015Article
- Normobaric hyperoxia-based neuroprotective therapies in ischemic stroke.Medical gas research · 2013Article
- Experimental models for assaying microvascular endothelial cell pathophysiology in stroke.Molecules (Basel, Switzerland) · 2010Review
- Pitavastatin strengthens the barrier integrity in primary cultures of rat brain endothelial cells.Cellular and molecular neurobiology · 2010Article
- Establishment of primary cultures of human brain microvascular endothelial cells to provide an in vitro cellular model of the blood-brain barrier.Nature protocols · 2010Article
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10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hemorrhagic transformation is a major complication associated with tissue plasminogen activator (tPA) therapy for ischemic stroke. We studied the effect of tPA on the blood-brain barrier (BBB) function with our in vitro monolayer model generated using rat brain microvascular endothelial cells subjected either to normoxia or to hypoxia/reoxygenation (H/R) with or without the administration of tPA. The barrier function was evaluated by the transendothelial electrical resistance (TEER), the permeability of sodium fluorescein and Evans' blue-albumin (EBA), and the uptake of lucifer yellow (LY). The permeability of sodium fluorescein and EBA was used as an index of paracellular and transcellular transport, respectively. The administration of tPA increased the permeability of EBA and the uptake of LY under normoxia. It enhanced the increase in the permeability of both sodium fluorescein and EBA, the decrease in the TEER, and the disruption in the expression of ZO-1 under H/R conditions. Administration of tPA could cause an increase in the transcellular transport under normoxia, and both the transcellular and paracellular transport of the BBB under H/R conditions in vitro. Even in humans, tPA may lead to an opening of the BBB under non-ischemic conditions and have an additional effect on the ischemia-induced BBB disruption.
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