ArticleComparative and functional genomics2005
Construction, verification and experimental use of two epitope-tagged collections of budding yeast strains.
Article in Comparative and functional genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Med9 Is a Novel eIF4E-Interacting Protein inInternational journal of molecular sciences · 2026Article
- Modular gene tagging inResearch square · 2026Article
- A comprehensive overview of yeast libraries and their role in advancing cell biology.The FEBS journal · 2026Review
- Modular gene tagging inbioRxiv : the preprint server for biology · 2026Article
- Review
- Article
- Using Baker's Yeast to Determine Functions of Novel Wolbachia (and Other Prokaryotic) Effectors.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Quantifying nonequilibrium dynamics and thermodynamics of cell fate decision making in yeast under pheromone induction.Biophysics reviews · 2023Article
- Article
- Article
- Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries.ACS synthetic biology · 2021Article
- Hsp90 interaction networks in fungi-tools and techniques.FEMS yeast research · 2021Review
- Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.Molecular biology of the cell · 2021Article
- Defining the role of the polyasparagine repeat domain of the S. cerevisiae transcription factor Azf1p.PloS one · 2021Article
- Conserved metabolite regulation of stress granule assembly via AdoMet.The Journal of cell biology · 2020Article
- A quantitative screen for metabolic enzyme structures reveals patterns of assembly across the yeast metabolic network.Molecular biology of the cell · 2019Article
- Improved discovery of genetic interactions using CRISPRiSeq across multiple environments.Genome research · 2019Article
- Thiol stress-dependent aggregation of the glycolytic enzyme triose phosphate isomerase in yeast and human cells.Molecular biology of the cell · 2019Article
- COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast.PloS one · 2019Article
- Gpn2 and Rba50 Directly Participate in the Assembly of the Rpb3 Subcomplex in the Biogenesis of RNA Polymerase II.Molecular and cellular biology · 2018Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major challenge in the post-genomic era is the development of experimental approaches to monitor the properties of proteins on a proteome-wide level. It would be particularly useful to systematically assay protein subcellular localization, post-translational modifications and protein-protein interactions, both at steady state and in response to environmental stimuli. Development of new reagents and methods will enhance our ability to do so efficiently and systematically. Here we describe the construction of two collections of budding yeast strains that facilitate proteome-wide measurements of protein properties. These collections consist of strains with an epitope tag integrated at the C-terminus of essentially every open reading frame (ORF), one with the tandem affinity purification (TAP) tag, and one with the green fluorescent protein (GFP) tag. We show that in both of these collections we have accurately tagged a high proportion of all ORFs (approximately 75% of the proteome) by confirming expression of the fusion proteins. Furthermore, we demonstrate the use of the TAP collection in performing high-throughput immunoprecipitation experiments. Building on these collections and the methods described in this paper, we hope that the yeast community will expand both the quantity and type of proteome level data available.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.