ArticleComparative and functional genomics2004
Comparative analysis of the testis and ovary transcriptomes in zebrafish by combining experimental and computational tools.
Article in Comparative and functional genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Article
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- Transcriptomic Responses ofInternational journal of molecular sciences · 2023Article
- Heat Shock Transcription Factor 2 Is Significantly Involved in Neurodegenerative Diseases, Inflammatory Bowel Disease, Cancer, Male Infertility, and Fetal Alcohol Spectrum Disorder: The Novel Mechanisms of Several Severe Diseases.International journal of molecular sciences · 2022Review
- Developmental RNA-Seq transcriptomics of haploid germ cells and spermatozoa uncovers novel pathways associated with teleost spermiogenesis.Scientific reports · 2022Article
- Shedding new light on early sex determination in zebrafish.Archives of toxicology · 2020Article
- Transcriptomic analysis of differentially expressed genes in the oviduct of Rhacophorus omeimontis provides insights into foam nest construction.BMC genomics · 2019Article
- Different expression patterns of sperm motility-related genes in testis of diploid and tetraploid cyprinid fish†.Biology of reproduction · 2017Article
- Global transcriptome analysis identifies regulated transcripts and pathways activated during oogenesis and early embryogenesis in Atlantic cod.Molecular reproduction and development · 2014Article
- Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones.PloS one · 2013Article
- Genomic approaches to study genetic and environmental influences on fish sex determination and differentiation.Marine biotechnology (New York, N.Y.) · 2012Review
- An ovary transcriptome for all maturational stages of the striped bass (Morone saxatilis), a highly advanced perciform fish.BMC research notes · 2012Article
- Global proteomics analysis of testis and ovary in adult zebrafish (Danio rerio).Fish physiology and biochemistry · 2011Article
- A microarray analysis of sex- and gonad-biased gene expression in the zebrafish: evidence for masculinization of the transcriptome.BMC genomics · 2009Article
- New insights into molecular pathways associated with flatfish ovarian development and atresia revealed by transcriptional analysis.BMC genomics · 2009Article
- Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.PloS one · 2008Article
- Identification of differentially expressed ovarian genes during primary and early secondary oocyte growth in coho salmon, Oncorhynchus kisutch.Reproductive biology and endocrinology : RB&E · 2008Article
- Profiling sex-biased gene expression during parthenogenetic reproduction in Daphnia pulex.BMC genomics · 2007Article
- Comparative genomics in cyprinids: common carp ESTs help the annotation of the zebrafish genome.BMC bioinformatics · 2006Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies on the zebrafish model have contributed to our understanding of several important developmental processes, especially those that can be easily studied in the embryo. However, our knowledge on late events such as gonad differentiation in the zebrafish is still limited. Here we provide an analysis on the gene sets expressed in the adult zebrafish testis and ovary in an attempt to identify genes with potential role in (zebra)fish gonad development and function. We produced 10,533 expressed sequence tags (ESTs) from zebrafish testis or ovary and downloaded an additional 23,642 gonad-derived sequences from the zebrafish EST database. We clustered these sequences together with over 13,000 kidney-derived zebrafish ESTs to study partial transcriptomes for these three organs. We searched for genes with gonad-specific expression by screening macroarrays containing at least 2600 unique cDNA inserts with testis-, ovary- and kidney-derived cDNA probes. Clones hybridizing to only one of the two gonad probes were selected, and subsequently screened with computational tools to identify 72 genes with potentially testis-specific and 97 genes with potentially ovary-specific expression, respectively. PCR-amplification confirmed gonad-specificity for 21 of the 45 clones tested (all without known function). Our study, which involves over 47,000 EST sequences and specialized cDNA arrays, is the first analysis of adult organ transcriptomes of zebrafish at such a scale. The study of genes expressed in adult zebrafish testis and ovary will provide useful information on regulation of gene expression in teleost gonads and might also contribute to our understanding of the development and differentiation of reproductive organs in vertebrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.