ArticleComparative and functional genomics2004
Comparative genomic assessment of novel broad-spectrum targets for antibacterial drugs.
Article in Comparative and functional genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Nanoscale Direct-to-Biology Optimization and Structural Insights into SelectiveJournal of medicinal chemistry · 2025Article
- Rapid accumulation of fluorophores and fast kill identify drugs with bactericidal effects against Gram-negative bacteria.Microbiology (Reading, England) · 2025Article
- Connecting tRNA Charging and Decoding through the Axis of Nucleotide Modifications at Position 37.Journal of molecular biology · 2025Review
- Antimicrobial resistance in Klebsiella pneumoniae: identification of bacterial DNA adenine methyltransferase as a novel drug target from hypothetical proteins using subtractive genomics.Genomics & informatics · 2022Article
- tRNA methylation resolves codon usage bias at the limit of cell viability.Cell reports · 2022Article
- The Transporter-Mediated Cellular Uptake and Efflux of Pharmaceutical Drugs and Biotechnology Products: How and Why Phospholipid Bilayer Transport Is Negligible in Real Biomembranes.Molecules (Basel, Switzerland) · 2021Review
- Loss ofeLife · 2021Article
- tRNA methylation: An unexpected link to bacterial resistance and persistence to antibiotics and beyond.Wiley interdisciplinary reviews. RNA · 2020Review
- MgACS catalysis · 2020Article
- tRNA Methylation Is a Global Determinant of Bacterial Multi-drug Resistance.Cell systems · 2019Article
- To Tie or Not to Tie? That Is the Question.Polymers · 2017Review
- Transfer RNA methyltransferases with a SpoU-TrmD (SPOUT) fold and their modified nucleosides in tRNA.Biomolecules · 2017Review
- Evolutionary Adaptation of the Essential tRNA Methyltransferase TrmD to the Signaling Molecule 3',5'-cAMP in Bacteria.The Journal of biological chemistry · 2017Article
- Methyl transfer by substrate signaling from a knotted protein fold.Nature structural & molecular biology · 2016Article
- CSAR 2014: A Benchmark Exercise Using Unpublished Data from Pharma.Journal of chemical information and modeling · 2016Article
- Structural basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Quantitative proteomic analysis of host--pathogen interactions: a study of Acinetobacter baumannii responses to host airways.BMC genomics · 2015Article
- Structural and functional features of enzymes of Mycobacterium tuberculosis peptidoglycan biosynthesis as targets for drug development.Tuberculosis (Edinburgh, Scotland) · 2015Review
- Identification of potential drug targets by subtractive genome analysis of Escherichia coli O157:H7: an in silico approach.Advances and applications in bioinformatics and chemistry : AABC · 2015Article
- A divalent metal ion-dependent N(1)-methyl transfer to G37-tRNA.Chemistry & biology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single and multiple resistance to antibacterial drugs currently in use is spreading, since they act against only a very small number of molecular targets; finding novel targets for anti-infectives is therefore of great importance. All protein sequences from three pathogens (Staphylococcus aureus, Mycobacterium tuberculosis and Escherichia coli O157:H7 EDL993) were assessed via comparative genomics methods for their suitability as antibacterial targets according to a number of criteria, including the essentiality of the protein, its level of sequence conservation, and its distribution in pathogens, bacteria and eukaryotes (especially humans). Each protein was scored and ranked based on weighted variants of these criteria in order to prioritize proteins as potential novel broad-spectrum targets for antibacterial drugs. A number of proteins proved to score highly in all three species and were robust to variations in the scoring system used. Sensitivity analysis indicated the quantitative contribution of each metric to the overall score. After further analysis of these targets, tRNA methyltransferase (trmD) and translation initiation factor IF-1 (infA) emerged as potential and novel antimicrobial targets very worthy of further investigation. The scoring strategy used might be of value in other areas of post-genomic drug discovery.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.