ArticleComparative and functional genomics2003
Functional genomics via metabolic footprinting: monitoring metabolite secretion by Escherichia coli tryptophan metabolism mutants using FT-IR and direct injection electrospray mass spectrometry.
Article in Comparative and functional genomics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Metabolic profiling analysis of cytochrome bMetabolomics : Official journal of the Metabolomic Society · 2026Article
- Plant and microbial sciences as key drivers in the development of metabolomics research.Proceedings of the National Academy of Sciences of the United States of America · 2023Review
- Metabolic Footprinting of Microbial Systems Based on Comprehensive In Silico Predictions of MS/MS Relevant Data.Metabolites · 2022Article
- Metabolic Fingerprint Analysis of CytochromeFrontiers in microbiology · 2022Article
- High-throughput dilution-based growth method enables time-resolved exo-metabolomics of Pseudomonas putida and Pseudomonas aeruginosa.Microbial biotechnology · 2021Article
- Extracellular Microbial Metabolomics: The State of the Art.Metabolites · 2017Review
- The metabolome 18 years on: a concept comes of age.Metabolomics : Official journal of the Metabolomic Society · 2016Review
- Dynamic exometabolome analysis reveals active metabolic pathways in non-replicating mycobacteria.Environmental microbiology · 2015Article
- Acclimation of metabolism to light in Arabidopsis thaliana: the glucose 6-phosphate/phosphate translocator GPT2 directs metabolic acclimation.Plant, cell & environment · 2015Article
- Profiling of spatial metabolite distributions in wheat leaves under normal and nitrate limiting conditions.Phytochemistry · 2015Article
- A metabolomics approach to stratify patients diagnosed with diabetes mellitus into excess or deficiency syndromes.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Metabolomic studies of human gastric cancer: review.World journal of gastroenterology · 2014Review
- Metabolomic analysis of the food-borne pathogenMetabolomics : Official journal of the Metabolomic Society · 2014Article
- Metabolomics in plants and humans: applications in the prevention and diagnosis of diseases.BioMed research international · 2013Review
- Emergent cooperation in microbial metabolism.Molecular systems biology · 2010Article
- Phenotypic characterization of Shewanella oneidensis MR-1 under aerobic and anaerobic growth conditions by using fourier transform infrared spectroscopy and high-performance liquid chromatography analyses.Applied and environmental microbiology · 2010Article
- Metabolic networks: how to identify key components in the regulation of metabolism and growth.Plant physiology · 2010Review
- Time-resolved metabolic footprinting for nonlinear modeling of bacterial substrate utilization.Applied and environmental microbiology · 2009Article
- Evaluation of predicted network modules in yeast metabolism using NMR-based metabolite profiling.Genome research · 2007Article
- Metabonomics techniques and applications to pharmaceutical research & development.Pharmaceutical research · 2006Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We sought to test the hypothesis that mutant bacterial strains could be discriminated from each other on the basis of the metabolites they secrete into the medium (their 'metabolic footprint'), using two methods of 'global' metabolite analysis (FT-IR and direct injection electrospray mass spectrometry). The biological system used was based on a published study of Escherichia coli tryptophan mutants that had been analysed and discriminated by Yanofsky and colleagues using transcriptome analysis. Wild-type strains supplemented with tryptophan or analogues could be discriminated from controls using FT-IR of 24 h broths, as could each of the mutant strains in both minimal and supplemented media. Direct injection electrospray mass spectrometry with unit mass resolution could also be used to discriminate the strains from each other, and had the advantage that the discrimination required the use of just two or three masses in each case. These were determined via a genetic algorithm. Both methods are rapid, reagentless, reproducible and cheap, and might beneficially be extended to the analysis of gene knockout libraries.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.