ArticlePloS one2008
In-cell biochemistry using NMR spectroscopy.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Ubiquitin's Conformational Heterogeneity as Discerned by Nuclear Magnetic Resonance Spectroscopy.Chembiochem : a European journal of chemical biology · 2024Review
- Multi-Dimensional Structure and Dynamics Landscape of Proteins in Mammalian Cells Revealed by In-Cell NMR.Angewandte Chemie (International ed. in English) · 2023Article
- Protein delivery to living cells by thermal stimulation for biophysical investigation.Scientific reports · 2022Article
- Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.Chemical reviews · 2022Review
- Controlling and quantifying protein concentration in Escherichia coli.Protein science : a publication of the Protein Society · 2019Article
- Interaction proteomics by using in-cell NMR spectroscopy.Journal of proteomics · 2019Review
- Potent Inhibitors of Mycobacterium tuberculosis Growth Identified by Using in-Cell NMR-based Screening.ACS chemical biology · 2018Article
- In-cell NMR: a topical review.IUCrJ · 2017Review
- A Unique Tool for Cellular Structural Biology: In-cell NMR.The Journal of biological chemistry · 2016Review
- In-cell ¹³C NMR spectroscopy for the study of intrinsically disordered proteins.Nature protocols · 2014Article
- The role of spartin and its novel ubiquitin binding region in DALIS occurrence.Molecular biology of the cell · 2014Article
- Real-time monitoring of New Delhi metallo-β-lactamase activity in living bacterial cells by 1H NMR spectroscopy.Angewandte Chemie (International ed. in English) · 2014Article
- Atomic-resolution monitoring of protein maturation in live human cells by NMR.Nature chemical biology · 2013Article
- Article
- In-cell NMR spectroscopy.Progress in nuclear magnetic resonance spectroscopy · 2011Review
- PTP1B targets the endosomal sorting machinery: dephosphorylation of regulatory sites on the endosomal sorting complex required for transport component STAM2.The Journal of biological chemistry · 2010Article
- Protein (19)F NMR in Escherichia coli.Journal of the American Chemical Society · 2010Article
- Screening of small molecule interactor library by using in-cell NMR spectroscopy (SMILI-NMR).Journal of medicinal chemistry · 2009Article
- Protein nuclear magnetic resonance under physiological conditions.Biochemistry · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biochemistry and structural biology are undergoing a dramatic revolution. Until now, mostly in vitro techniques have been used to study subtle and complex biological processes under conditions usually remote from those existing in the cell. We developed a novel in-cell methodology to post-translationally modify interactor proteins and identify the amino acids that comprise the interaction surface of a target protein when bound to the post-translationally modified interactors. Modifying the interactor proteins causes structural changes that manifest themselves on the interacting surface of the target protein and these changes are monitored using in-cell NMR. We show how Ubiquitin interacts with phosphorylated and non-phosphorylated components of the receptor tyrosine kinase (RTK) endocytic sorting machinery: STAM2 (Signal-transducing adaptor molecule), Hrs (Hepatocyte growth factor regulated substrate) and the STAM2-Hrs heterodimer. Ubiquitin binding mediates the processivity of a large network of interactions required for proper functioning of the RTK sorting machinery. The results are consistent with a weakening of the network of interactions when the interactor proteins are phosphorylated. The methodology can be applied to any stable target molecule and may be extended to include other post-translational modifications such as ubiquitination or sumoylation, thus providing a long-awaited leap to high resolution in cell biochemistry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.