ArticleInfection and immunity2008
Salmonella enterica serovar Typhimurium NiFe uptake-type hydrogenases are differentially expressed in vivo.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists.Microbiology and molecular biology reviews : MMBR · 2020Review
- Dissection of the Hydrogen Metabolism of the Enterobacterium Trabulsiella guamensis: Identification of a Formate-Dependent and Essential Formate Hydrogenlyase Complex Exhibiting Phylogenetic Similarity to Complex I.Journal of bacteriology · 2019Article
- The structure of hydrogenase-2 fromThe Biochemical journal · 2018Article
- Article
- First in Vivo Batrachochytrium dendrobatidis Transcriptomes Reveal Mechanisms of Host Exploitation, Host-Specific Gene Expression, and Expressed Genotype Shifts.G3 (Bethesda, Md.) · 2017Article
- Structure-function analyses of metal-binding sites of HypA reveal residues important for hydrogenase maturation in Helicobacter pylori.PloS one · 2017Article
- Identification of a stable complex between a [NiFe]-hydrogenase catalytic subunit and its maturation protease.FEBS letters · 2017Article
- Anaerobic Formate and Hydrogen Metabolism.EcoSal Plus · 2016Review
- Host hydrogen rather than that produced by the pathogen is important for Salmonella enterica serovar Typhimurium virulence.Infection and immunity · 2015Article
- Transcriptional profile of Salmonella enterica subsp. enterica serovar Weltevreden during alfalfa sprout colonization.Microbial biotechnology · 2014Article
- How the structure of the large subunit controls function in an oxygen-tolerant [NiFe]-hydrogenase.The Biochemical journal · 2014Article
- The growth and survival of Mycobacterium smegmatis is enhanced by co-metabolism of atmospheric H2.PloS one · 2014Article
- Salmonella Typhimurium strain ATCC14028 requires H2-hydrogenases for growth in the gut, but not at systemic sites.PloS one · 2014Article
- Central role of liver in anticancer and radioprotective activities of Toll-like receptor 5 agonist.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Roles of H2 uptake hydrogenases in Shigella flexneri acid tolerance.Microbiology (Reading, England) · 2012Article
- In vivo expression of Salmonella enterica serotype Typhi genes in the blood of patients with typhoid fever in Bangladesh.PLoS neglected tropical diseases · 2011Article
- Role of the Hya hydrogenase in recycling of anaerobically produced H2 in Salmonella enterica serovar Typhimurium.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica serovar Typhimurium, a common enteric pathogen, possesses three NiFe uptake-type hydrogenases. The results from mouse infection studies suggest that the H(2) oxidation capacity provided by these hydrogenases is important for virulence. Since the three enzymes are similar in structure and function, it may be expected that they are utilized under different locations and times during an infection. A recombination-based method to examine promoter activity in vivo (RIVET) was used to determine hydrogenase gene expression in macrophages, polymorphonuclear leukocyte (PMN)-like cells, and a mouse model of salmonellosis. The hyd and hya promoters showed increased expression in both murine macrophages and human PMN-like cells compared to that in the medium-only controls. Quantitative reverse transcription-PCR results suggested that hyb is also expressed in phagocytes. A nonpolar hya mutant was compromised for survival in macrophages compared to the wild type. This may be due to lower tolerance to acid stress, since the hya mutant was much more acid sensitive than the wild type. In addition, hya mutant cells were internalized by macrophages the same as wild-type cells. Mouse studies (RIVET) indicate that hyd is highly expressed in the liver and spleen early during infection but is expressed poorly in the ileum in infected animals. Late in the infection, the hyd genes were expressed at high levels in the ileum as well as in the liver and spleen. The hya genes were expressed at low levels in all locations tested. These results suggest that the hydrogenases are used to oxidize hydrogen in different stages of an infection.
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