ArticleThe Journal of biological chemistry2008
Importance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 79 citations in OpenAlex.
- The structural chemistry and biosynthesis of chlorophylls.RSC chemical biology · 2026Review
- Article
- Chlorophyll biosynthesis under the control of arginine metabolism.Cell reports · 2023Article
- GUN4 appeared early in cyanobacterial evolution.PNAS nexus · 2023Article
- Impact of Porphyrin Binding to GENOMES UNCOUPLED 4 on Tetrapyrrole BiosynthesisFrontiers in plant science · 2022Article
- Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4.Protein science : a publication of the Protein Society · 2021Article
- Bilin-dependent regulation of chlorophyll biosynthesis by GUN4.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Evolution of Ycf54-independent chlorophyll biosynthesis in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mutations of theFrontiers in plant science · 2021Article
- Cell wall surface layer (S-layer) promotes colony formation in Microcystis: comparison of S-layer characteristics between colonial and unicellular forms of Microcystis and function conformation.Environmental science and pollution research international · 2020Article
- Role of diversity-generating retroelements for regulatory pathway tuning in cyanobacteria.BMC genomics · 2020Article
- Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803.Bioscience reports · 2020Review
- A Suppressor Mutation Partially Reverts theFrontiers in plant science · 2019Article
- TheFrontiers in plant science · 2017Article
- Phosphorylation of GENOMES UNCOUPLED 4 Alters Stimulation of Mg Chelatase Activity in Angiosperms.Plant physiology · 2016Article
- GUN4-Protoporphyrin IX Is a Singlet Oxygen Generator with Consequences for Plastid Retrograde Signaling.The Journal of biological chemistry · 2016Article
- Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803.Frontiers in plant science · 2016Article
- Mutation of Gly195 of the ChlH Subunit of Mg-chelatase Reduces Chlorophyll and Further Disrupts PS II Assembly in a Ycf48-Deficient Strain of Synechocystis sp. PCC 6803.Frontiers in plant science · 2016Article
- Porphyrin Binding to Gun4 Protein, Facilitated by a Flexible Loop, Controls Metabolite Flow through the Chlorophyll Biosynthetic Pathway.The Journal of biological chemistry · 2015Article
- Lack of Phosphatidylglycerol Inhibits Chlorophyll Biosynthesis at Multiple Sites and Limits Chlorophyllide Reutilization in Synechocystis sp. Strain PCC 6803.Plant physiology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gun4 is a porphyrin-binding protein that activates magnesium chelatase, a multimeric enzyme catalyzing the first committed step in chlorophyll biosynthesis. In plants, GUN4 has been implicated in plastid-to-nucleus retrograde signaling processes that coordinate both photosystem II and photosystem I nuclear gene expression with chloroplast function. In this work we present the functional analysis of Gun4 from the cyanobacterium Synechocystis sp. PCC 6803. Affinity co-purification of the FLAG-tagged Gun4 with the ChlH subunit of the magnesium chelatase confirmed the association of Gun4 with the enzyme in cyanobacteria. Inactivation of the gun4 gene abolished photoautotrophic growth of the resulting gun4 mutant strain that exhibited a decreased activity of magnesium chelatase. Consequently, the cellular content of chlorophyll-binding proteins was highly inadequate, especially that of proteins of photosystem II. Immunoblot analyses, blue native polyacrylamide gel electrophoresis, and radiolabeling of the membrane protein complexes suggested that the availability of the photosystem II antenna protein CP47 is a limiting factor for the photosystem II assembly in the gun4 mutant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.