ArticleIn vitro cellular & developmental biology. Animal
Standardization of a colorimetric method to quantify growth and metabolic activity of Wolbachia-infected mosquito cells.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Knockout of caspase-7 gene improves the expression of recombinant protein in CHO cell line through the cell cycle arrest in G2/M phase.Biological research · 2022Article
- Discovery of mosquitocides from fungal extracts through a high-throughput cytotoxicity-screening approach.Parasites & vectors · 2021Article
- Growth and Maintenance ofInsects · 2021Review
- Assessment of mitotically inactivated mosquito cell feeder layers produced with mitomycin C.In vitro cellular & developmental biology. Animal · 2021Article
- Effects of mimosine on Wolbachia in mosquito cells: cell cycle suppression reduces bacterial abundance.In vitro cellular & developmental biology. Animal · 2015Article
- The oxidizing agent, paraquat, is more toxic to Wolbachia than to mosquito host cells.In vitro cellular & developmental biology. Animal · 2013Article
- Wolbachia from the planthopper Laodelphax striatellus establishes a robust, persistent, streptomycin-resistant infection in clonal mosquito cells.In vitro cellular & developmental biology. Animal · 2013Article
- Gene expression profiles in human HepG2 cells treated with extracts of the Tamarindus indica fruit pulp.Genes & nutrition · 2010Article
- Proteasome activity in a naïve mosquito cell line infected with Wolbachia pipientis wAlbB.In vitro cellular & developmental biology. Animal · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Aedes albopictus Aa23 cell line, which is persistently infected with Wolbachia pipientis strain wAlbB, tends to grow as aggregated clusters of cells that are difficult to disperse for conventional quantification based on cell number. We used A. albopictus C7-10 cells to validate conversion of methylthiazole tetrazolium (MTT) to a colored formazan product with respect to incubation time, cell number over a 40-fold range, and metabolic activity as cells enter stationary phase. Using this assay, we showed that the doubling time of Aa23 cells increases from about 45 h early after plating to more than 70 h as the cells reach stationary levels. Growth of Aa23 cells proceeds at similar rates in the presence or absence of tetracycline concentrations that decrease the abundance of Wolbachia. Insofar as the MTT assay reflects mitochondrial function, our results indicate that, in Aa23 cells, abundance of intracellular Wolbachia has no measurable effect on mitochondrial activity in the presence of tetracycline.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.