ArticlePlant physiology2008
Geminivirus-mediated gene silencing from Cotton leaf crumple virus is enhanced by low temperature in cotton.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 147 citations in OpenAlex.
- Plant viruses as next-generation vectors for transgene-free genome editing, gene regulation, and rapid crop improvement.Plant molecular biology · 2026Review
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- APathogens (Basel, Switzerland) · 2025Article
- Establishment of a TRV-based virus-induced gene silencing system using the vacuum infiltration in Tartary buckwheat.Plant cell reports · 2025Article
- Genome-wide analysis and functional validation of the GH1 gene family in cotton under salt stress.Functional & integrative genomics · 2025Article
- Genome-wide identification of VOZ gene family in ten cotton species and the function analysis of GhVOZ2 involved in heat stress response.BMC genomics · 2025Article
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- Genome-wide identification and analysis of the cotton ALDH gene family.BMC genomics · 2024Article
- Unveiling the dynamic relationship of viruses and/or symbiotic bacteria with plant resilience in abiotic stress.Stress biology · 2024Review
- Applications of Virus-Induced Gene Silencing in Cotton.Plants (Basel, Switzerland) · 2024Review
- Establishment and application of a root wounding-immersion method for efficient virus-induced gene silencing in plants.Frontiers in plant science · 2024Article
- Cotton Meristem Transcriptomes: Constructing an RNA-Seq Pipeline to Explore Crop Architecture Regulation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Virus-Induced Gene Silencing in the Tea Plant (Plants (Basel, Switzerland) · 2023Article
- Chitinase of Trichoderma longibrachiatum for control of Aphis gossypii in cotton plants.Scientific reports · 2023Article
- Altered expression of SELF-PRUNING disrupts homeostasis and facilitates signal delivery to meristems.Plant physiology · 2023Article
- Article
- Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.International journal of molecular sciences · 2023Review
- Cotton (International journal of molecular sciences · 2022Article
- Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Plants (Basel, Switzerland) · 2022Article
- Dynamic Expression, Differential Regulation and Functional Diversity of the CNGC Family Genes in Cotton.International journal of molecular sciences · 2022Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A silencing vector for cotton (Gossypium hirsutum) was developed from the geminivirus Cotton leaf crumple virus (CLCrV). The CLCrV coat protein gene was replaced by up to 500 bp of DNA homologous to one of two endogenous genes, the magnesium chelatase subunit I gene (ChlI) or the phytoene desaturase gene (PDS). Cotyledons of cotton cultivar 'Deltapine 5415' bombarded with the modified viral vectors manifested chlorosis due to silencing of either ChlI or PDS in approximately 70% of inoculated plants after 2 to 3 weeks. Use of the green fluorescence protein gene showed that replication of viral DNA was restricted to vascular tissue and that the viral vector could transmit to leaves, roots, and the ovule integument from which fibers originate. Temperature had profound effects on vector DNA accumulation and the spread of endogenous gene silencing. Consistent with reports that silencing against viruses increases at higher temperatures, plants grown at a 30 degrees C/26 degrees C day/night cycle had a greater than 10-fold reduction in viral DNA accumulation compared to plants grown at 22 degrees C/18 degrees C. However, endogenous gene silencing decreased at 30 degrees C/26 degrees C. There was an approximately 7 d delay in the onset of gene silencing at 22 degrees C/18 degrees C, but silencing was extensive and persisted throughout the life of the plant. The extent of silencing in new growth could be increased or decreased by changing temperature regimes at various times following the onset of silencing. Our experiments establish the use of the CLCrV silencing vector to study gene function in cotton and show that temperature can have a major impact on the extent of geminivirus-induced gene silencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.