ArticleAnnals of botany2008
Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development.
Article in Annals of botany, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Wheat DOF transcription factors TaSAD and WPBF regulate glutenin gene expression in cooperation with SPA.PloS one · 2023Article
- The α-Gliadins in Bread Wheat: Effect of Nitrogen Treatment on the Expression of the Major Celiac Disease Immunogenic Complex in Two RNAi Low-Gliadin Lines.Frontiers in plant science · 2021Article
- Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain.PloS one · 2021Article
- Over-ExpressingInternational journal of molecular sciences · 2020Article
- Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Comprehensive comparison and applications of different sections in investigating the microstructure and histochemistry of cereal kernels.Plant methods · 2020Review
- Active DNA demethylation: mechanism and role in plant development.Plant cell reports · 2018Review
- Article
- Comprehensive molecular characterization of the α/β-gliadin multigene family in hexaploid wheat.Molecular genetics and genomics : MGG · 2016Article
- Conserved cis-regulatory modules in promoters of genes encoding wheat high-molecular-weight glutenin subunits.Frontiers in plant science · 2014Article
- Quantitative and qualitative differences in celiac disease epitopes among durum wheat varieties identified through deep RNA-amplicon sequencing.BMC genomics · 2013Article
- Association study of wheat grain protein composition reveals that gliadin and glutenin composition are trans-regulated by different chromosome regions.Journal of experimental botany · 2013Article
- Targeted modification of wheat grain protein to reduce the content of celiac causing epitopes.Functional & integrative genomics · 2012Review
- Delivery of prolamins to the protein storage vacuole in maize aleurone cells.The Plant cell · 2011Article
- Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin.BMC plant biology · 2009Article
- Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci.BMC genomics · 2009Article
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
BACKGROUND AND
aimsAlpha-gliadin proteins are important for the industrial quality of bread wheat flour, but they also contain many epitopes that can trigger celiac (coeliac) disease (CD). The B-genome-encoded alpha-gliadin genes, however, contain very few epitopes. Controlling alpha-gliadin gene expression in wheat requires knowledge on the processes of expression and deposition of alpha-gliadin protein during wheat grain development.
methodsA 592-bp fragment of the promotor of a B-genome-encoded alpha-gliadin gene driving the expression of a GUS reporter gene was transformed into wheat. A large number of transgenic lines were used for data collection. GUS staining was used to determine GUS expression during wheat kernel development, and immunogold labelling and tissue printing followed by staining with an alpha-gliadin-specific antibody was used to detect alpha-gliadin protein deposited in developing wheat kernels. The promoter sequence was screened for regulatory motifs and compared to other available alpha-gliadin promoter sequences. KEY
resultsGUS expression was detected primarily in the cells of the starchy endosperm, notably in the subaleurone layer but also in the aleurone layer. The alpha-gliadin promoter was active from 11 days after anthesis (DAA) until maturity, with an expression similar to that of a 326-bp low molecular weight (LMW) subunit gene promoter reported previously. An alpha-gliadin-specific antibody detected alpha-gliadin protein in protein bodies in the starchy endosperm and in the subaleurone layer but, in contrast to the promoter activity, no alpha-gliadin was detected in the aleurone cell layer. Sequence comparison showed differences in regulatory elements between the promoters of alpha-gliadin genes originating from different genomes (A and B) of bread wheat both in the region used here and upstream.
conclusionsThe results suggest that additional regulator elements upstream of the promoter region used may specifically repress expression in the aleurone cell layer. Observed differences in expression regulator motifs between the alpha-gliadin genes on the different genomes (A and B) of bread wheat leads to a better understanding how alpha-gliadin expression can be controlled.
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