ArticleEukaryotic cell2008
Rho5p is involved in mediating the osmotic stress response in Saccharomyces cerevisiae, and its activity is regulated via Msi1p and Npr1p by phosphorylation and ubiquitination.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 31 citations in OpenAlex.
- Construction of yeast with extremely high 2,3-butanediol tolerance by introducing point and structural mutations and partial elucidation of the mechanism of 2,3-butanediol tolerance.Applied microbiology and biotechnology · 2025Article
- The small GTPase Rho5-Yet another player in yeast glucose signaling.PLoS genetics · 2025Article
- Biological Importance of Complex Sphingolipids and Their Structural Diversity in Budding YeastInternational journal of molecular sciences · 2024Review
- Review
- Dual RNA-Seq Profiling Unveils Mycoparasitic Activities ofMicrobiology spectrum · 2023Article
- The Intracellular Distribution of the Small GTPase Rho5 and Its Dimeric Guanidine Nucleotide Exchange Factor Dck1/Lmo1 Determine Their Function in Oxidative Stress Response.International journal of molecular sciences · 2022Article
- The NPR/Hal family of protein kinases in yeasts: biological role, phylogeny and regulation under environmental challenges.Computational and structural biotechnology journal · 2022Review
- Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors.AMB Express · 2021Article
- New Aspects of Invasive Growth Regulation Identified by Functional Profiling of MAPK Pathway Targets inGenetics · 2020Article
- Analysis of Functional Domains in Rho5, the Yeast Homolog of Human Rac1 GTPase, in Oxidative Stress Response.International journal of molecular sciences · 2019Article
- Genome-Wide Studies of Rho5-Interacting Proteins That Are Involved in Oxidant-Induced Cell Death in Budding Yeast.G3 (Bethesda, Md.) · 2019Article
- Protection mechanisms against aberrant metabolism of sphingolipids in budding yeast.Current genetics · 2018Review
- The Small Yeast GTPase Rho5 and Its Dimeric GEF Dck1/Lmo1 Respond to Glucose Starvation.International journal of molecular sciences · 2018Article
- Membrane Phosphoproteomics of Yeast Early Response to Acetic Acid: Role of Hrk1 Kinase and Lipid Biosynthetic Pathways, in Particular Sphingolipids.Frontiers in microbiology · 2017Article
- Article
- The zinc cluster protein Sut1 contributes to filamentation in Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling.Molecular biology of the cell · 2013Article
- Role of Cryptococcus neoformans Rho1 GTPases in the PKC1 signaling pathway in response to thermal stress.Eukaryotic cell · 2013Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Alpha-arrestins Aly1 and Aly2 regulate intracellular trafficking in response to nutrient signaling.Molecular biology of the cell · 2010Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small GTPases of the Rho family act as molecular switches, and modulation of the GTP-bound state of Rho proteins is a well-characterized means of regulating their signaling activity in vivo. In contrast, the regulation of Rho-type GTPases by posttranslational modifications is poorly understood. Here, we present evidence of the control of the Saccharomyces cerevisiae Rho-type GTPase Rho5p by phosphorylation and ubiquitination. Rho5p binds to Ste50p, and the expression of the activated RHO5(Q91H) allele in an Deltaste50 strain is lethal under conditions of osmotic stress. An overexpression screen identified RGD2 and MSI1 as being high-copy suppressors of the osmotic sensitivity of this lethality. Rgd2p had been identified as being a possible Rho5p GTPase-activating protein based on an in vitro assay; this result supports its function as a regulator of Rho5p activity in vivo. MSI1 was previously identified as being a suppressor of hyperactive Ras/cyclic AMP signaling, where it antagonizes Npr1p kinase activity and promotes ubiquitination. Here, we show that Msi1p also acts via Npr1p to suppress activated Rho5p signaling. Rho5p is ubiquitinated, and its expression is lethal in a strain that is compromised for proteasome activity. These data identify Rho5p as being a target of Msi1p/Npr1p regulation and describe a regulatory circuit involving phosphorylation and ubiquitination.
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