ArticleApplied and environmental microbiology2008
Isolation of predation-deficient mutants of Bdellovibrio bacteriovorus by using transposon mutagenesis.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Functional analysis of natural variation in the RNA-binding protein CsrA across the bacterial domain predicts regulatory activity.Nucleic acids research · 2026Article
- Using AlphaFold-Multimer to study novel protein-protein interactions of predation essential hypothetical proteins inFrontiers in bioinformatics · 2025Article
- Distinct dynamics and proximity networks of hub proteins at the prey-invading cell pole in a predatory bacterium.Journal of bacteriology · 2024Article
- Multicopy expression of sigma factor RpoH reduces prodigiosin biosynthesis in Serratia marcescens FS14.Antonie van Leeuwenhoek · 2023Article
- Moving toward a better understanding of the model bacterial predatorMicrobiology (Reading, England) · 2023Article
- Fitness Costs of Tigecycline Resistance inAntibiotics (Basel, Switzerland) · 2022Article
- Insight into the Possible Use of the PredatorNutrients · 2020Review
- Second messengers and divergent HD-GYP phosphodiesterases regulate 3',3'-cGAMP signaling.Molecular microbiology · 2020Article
- Biotechnological Potential ofFrontiers in microbiology · 2020Review
- High-Throughput Analysis of Gene Function in the Bacterial Predator Bdellovibrio bacteriovorus.mBio · 2019Article
- Identification and Characterization of Differentially-Regulated Type IVb Pilin Genes Necessary for Predation in Obligate Bacterial Predators.Scientific reports · 2017Article
- Identification of α-amylase by random and specific mutagenesis of Texcoconibacillus texcoconensis 13CCT strain isolated from extreme alkaline-saline soil of the former Lake Texcoco (Mexico).Folia microbiologica · 2014Article
- Identification of genes essential for prey-independent growth of Bdellovibrio bacteriovorus HD100.Journal of bacteriology · 2011Article
- Assessment of the mobilizable vector plasmids pSUP202 and pSUP404.2 as genetic tools for the predatory bacterium Bdellovibrio bacteriovorus.Current microbiology · 2011Article
- Article
- A new method for isolating host-independent variants of Bdellovibrio bacteriovorus using E. coli auxotrophs.The open microbiology journal · 2009Article
- Effective mutagenesis of Vibrio fischeri by using hyperactive mini-Tn5 derivatives.Applied and environmental microbiology · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The availability of the complete genome sequence of Bdellovibrio bacteriovorus provides an opportunity for investigating genes that play a significant role in predation. Using two independently derived facultatively predatory Bdellovibrio strains, we have designed a method to cultivate and screen transposon insertion mutants in 96-well microtiter dishes. Transposon insertion mutants were produced by introducing the plasposon pRL27, which carries a mini-Tn5. Mutants have been screened for predatory activity using 96-well plates. Seventeen independent nonpredatory mutants have been isolated, and DNA flanking the insertion has been sequenced. BLAST analysis revealed that most of these interrupted DNA sequences do not code for known proteins or functions. Two of the inactivated genes were analyzed further: one was found to code for a putative serine protease and the other a probable protein involved in secretion through the outer membrane. The methods described here are the first for the generation and isolation of predation-deficient mutants using random-transposon-insertion mutagenesis. As more mutants are isolated and their gene products analyzed, more light will be shed on how this predator carries out its exclusive life processes and perhaps how these products, or the organism itself, can be used for therapeutic, agricultural, and/or other purposes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.