ArticleApplied and environmental microbiology2008
Real-time PCR detection of pathogenic microorganisms in roof-harvested rainwater in Southeast Queensland, Australia.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 185 citations in OpenAlex.
- Quantitative microbial risk assessment of pathogen exposure from rainwater used in high-pressure vehicle washing.Journal of water and health · 2025Article
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- Metabolically active bacteria detected with click chemistry in low organic matter rainwater.PloS one · 2023Article
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- Characterization of roof runoff microbial quality in four U.S. cities with varying climate and land use characteristics.Water research · 2022Article
- Detection ofMicroorganisms · 2022Review
- Risk Factors Associated with Diarrheal Episodes in an Agricultural Community in Nam Dinh Province, Vietnam: A Prospective Cohort Study.International journal of environmental research and public health · 2022Article
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- Human Pathogenic Bacteria Detected in Rainwater: Risk Assessment and Correlation to Microbial Source Tracking Markers and Traditional Indicators.Frontiers in microbiology · 2021Article
- Quantitative LAMP and PCR Detection ofInternational journal of food science · 2020Article
- Exploration of Anti-infectives From Mangrove-DerivedFrontiers in microbiology · 2020Article
- Evaluation of essential oils and a prebiotic for newborn dairy calves.Translational animal science · 2020Article
- Novel Bacteriophages Capable of Disrupting Biofilms From Clinical Strains ofFrontiers in microbiology · 2020Article
- A review on microbial contaminants in stormwater runoff and outfalls: Potential health risks and mitigation strategies.The Science of the total environment · 2019Review
- Relative proportions of E. coli and Enterococcus spp. may be a good indicator of potential health risks associated with the use of roof harvested rainwater stored in tanks.Environmental monitoring and assessment · 2018Article
- Abundance of Naegleria fowleri in roof-harvested rainwater tank samples from two continents.Environmental science and pollution research international · 2018Article
- Microfluidic quantification of multiple enteric and opportunistic bacterial pathogens in roof-harvested rainwater tank samples.Environmental monitoring and assessment · 2018Article
- Assessment of the Physicochemical and Heavy Metal Qualities of Rooftop Harvested Rainwater in a Rural Community.Global challenges (Hoboken, NJ) · 2017Article
- Quality assessment and public health status of harvested rainwater in a peri-urban community in Edo State of Nigeria.Environmental monitoring and assessment · 2017Article
- Detection of Intestinal Pathogens in River, Shore, and Drinking Water in Lima, Peru.Journal of genomics · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, the microbiological quality of roof-harvested rainwater was assessed by monitoring the concentrations of Escherichia coli, enterococci, Clostridium perfringens, and Bacteroides spp. in rainwater obtained from tanks in Southeast Queensland, Australia. Samples were also tested using real-time PCR (with SYBR Green I dye) for the presence of potential pathogenic microorganisms. Of the 27 rainwater samples tested, 17 (63%), 21 (78%), 13 (48%), and 24 (89%) were positive for E. coli, enterococci, C. perfringens, and Bacteroides spp., respectively. Of the 27 samples, 11 (41%), 7 (26%), 4 (15%), 3 (11%), and 1 (4%) were PCR positive for the Campylobacter coli ceuE gene, the Legionella pneumophila mip gene, the Aeromonas hydrophila lip gene, the Salmonella invA gene, and the Campylobacter jejuni mapA gene. Of the 21 samples tested, 4 (19%) were positive for the Giardia lamblia beta-giardin gene. The binary logistic regression model indicated a positive correlation (P < 0.02) between the presence/absence of enterococci and A. hydrophila. In contrast, the presence/absence of the remaining potential pathogens did not correlate with traditional fecal indicators. The poor correlation between fecal indicators and potential pathogens suggested that fecal indicators may not be adequate to assess the microbiological quality of rainwater and consequent health risk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.