ArticleNucleic acids research2008
Spatial configuration of the chicken alpha-globin gene domain: immature and active chromatin hubs.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
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- Investigation of the Basic Steps in the Chromosome Conformation Capture Procedure.Frontiers in genetics · 2021Article
- MYC as a driver of stochastic chromatin networks: implications for the fitness of cancer cells.Nucleic acids research · 2020Article
- Enhancer functions in three dimensions: beyond the flat world perspective.F1000Research · 2018Review
- Activation of the alpha-globin gene expression correlates with dramatic upregulation of nearby non-globin genes and changes in local and large-scale chromatin spatial structure.Epigenetics & chromatin · 2017Article
- 3D genomics imposes evolution of the domain model of eukaryotic genome organization.Chromosoma · 2017Review
- Differential contribution of cis-regulatory elements to higher order chromatin structure and expression of the CFTR locus.Nucleic acids research · 2016Article
- The clustering of CpG islands may constitute an important determinant of the 3D organization of interphase chromosomes.Epigenetics · 2014Article
- A long non-coding RNA promotes full activation of adult gene expression in the chicken α-globin domain.Epigenetics · 2014Article
- CTCF demarcates chicken embryonic α-globin gene autonomous silencing and contributes to adult stage-specific gene expression.Epigenetics · 2013Article
- Disclosure of a structural milieu for the proximity ligation reveals the elusive nature of an active chromatin hub.Nucleic acids research · 2013Article
- Spatial organization of the chicken beta-globin gene domain in erythroid cells of embryonic and adult lineages.Epigenetics & chromatin · 2012Article
- RUNX1 regulates the CD34 gene in haematopoietic stem cells by mediating interactions with a distal regulatory element.The EMBO journal · 2011Article
- The BCL2 gene is regulated by a special AT-rich sequence binding protein 1-mediated long range chromosomal interaction between the promoter and the distal element located within the 3'-UTR.Nucleic acids research · 2011Article
- An insulator embedded in the chicken α-globin locus regulates chromatin domain configuration and differential gene expression.Nucleic acids research · 2011Article
- Mapping of the nuclear matrix-bound chromatin hubs by a new M3C experimental procedure.Nucleic acids research · 2010Article
- TMEM8 - a non-globin gene entrapped in the globin web.Nucleic acids research · 2009Article
- The organization in micro-loops of an extended fragment of chicken chromosome 14, including the alpha globin gene cluster in the erythroid cells.Acta naturae · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spatial configuration of the chicken alpha-globin gene domain in erythroid and lymphoid cells was studied by using the Chromosome Conformation Capture (3C) approach. Real-time PCR with TaqMan probes was employed to estimate the frequencies of cross-linking of different restriction fragments within the domain. In differentiated cultured erythroblasts and in 10-day chick embryo erythrocytes expressing 'adult' alpha(A) and alpha(D) globin genes the following elements of the domain were found to form an 'active' chromatin hub: upstream Major Regulatory Element (MRE), -9 kb upstream DNase I hypersensitive site (DHS), -4 kb upstream CpG island, alpha(D) gene promoter and the downstream enhancer. The alpha(A) gene promoter was not present in the 'active' chromatin hub although the level of alpha(A) gene transcription exceeded that of the alpha(D) gene. Formation of the 'active' chromatin hub was preceded by the assembly of multiple incomplete hubs containing MRE in combination with either -9 kb DHS or other regulatory elements of the domain. These incomplete chromatin hubs were present in proliferating cultured erythroblasts which did not express globin genes. In lymphoid cells only the interaction between the alpha(D) promoter and the CpG island was detected.
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