ArticleProceedings of the National Academy of Sciences of the United States of America2008
Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- JANUS, a spliceosome-associated protein, promotes miRNA biogenesis in Arabidopsis.Nucleic acids research · 2024Article
- A Note on the Effects of Linear Topology Preservation in Monte Carlo Simulations of Knotted Proteins.International journal of molecular sciences · 2022Article
- Phf5a regulates DNA repair in class switch recombination via p400 and histone H2A variant deposition.The EMBO journal · 2021Article
- Rewards of divergence in sequences, 3-D structures and dynamics of yeast and human spliceosome SF3b complexes.Current research in structural biology · 2021Article
- Structural and functional modularity of the U2 snRNP in pre-mRNA splicing.Critical reviews in biochemistry and molecular biology · 2019Review
- Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain.RNA (New York, N.Y.) · 2017Article
- Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.Nature communications · 2017Article
- Structural and mechanistic insights into human splicing factor SF3b complex derived using an integrated approach guided by the cryo-EM density maps.RNA biology · 2016Article
- Classification of the treble clef zinc finger: noteworthy lessons for structure and function evolution.Scientific reports · 2016Article
- Folding analysis of the most complex Stevedore's protein knot.Scientific reports · 2016Article
- In Search of Functional Advantages of Knots in Proteins.PloS one · 2016Article
- The UBR-box and its relationship to binuclear RING-like treble clef zinc fingers.Biology direct · 2015Article
- Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.PLoS genetics · 2015Article
- A knot in the protein structure - probing the near-infrared fluorescent protein iRFP designed from a bacterial phytochrome.The FEBS journal · 2014Article
- Polymer uncrossing and knotting in protein folding, and their role in minimal folding pathways.PloS one · 2013Article
- A topological framework for the computation of the HOMFLY polynomial and its application to proteins.PloS one · 2011Article
- A Stevedore's protein knot.PLoS computational biology · 2010Article
- Sequence-specific size, structure, and stability of tight protein knots.Biophysical journal · 2009Article
- Zinc finger structure determination by NMR: Why zinc fingers can be a handful.Progress in nuclear magnetic resonance spectroscopyReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Rds3p, a component of the U2 snRNP subcomplex SF3b, is essential for pre-mRNA splicing and is extremely well conserved in all eukaryotic species. We report here the solution structure of Rds3p, which reveals an unusual knotted fold unrelated to previously known knotted proteins. Rds3p has a triangular shape with a GATA-like zinc finger at each vertex. Pairs of cysteines contributing to each finger are arranged nonsequentially in a permuted arrangement reminiscent of domain-swapping but which here involves segments of subdomains within a single chain. We suggest that the structure arose through a process of segment swapping after gene duplication. The fingers are connected through beta-strands and loops, forming an overall topology strongly resembling a "triquetra knot." The conservation and surface properties of Rds3p suggest that it functions as a platform for protein assembly within the multiprotein SF3b complex of U2 snRNP. The recombinant protein used for structure determination is biologically active, as it restores splicing activity in a yeast splicing extract depleted of native Rds3p.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.