ArticleProceedings of the National Academy of Sciences of the United States of America2008
Subnanometer-resolution electron cryomicroscopy-based domain models for the cytoplasmic region of skeletal muscle RyR channel.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 109 citations in OpenAlex.
- Ligand-induced activation of RyR1 in native membranes.Nature communications · 2026Article
- Connexin hemichannels as candidate targets for cardioprotective and anti-arrhythmic treatments.The Journal of clinical investigation · 2023Review
- Structure and Function of the Human Ryanodine Receptors and Their Association with Myopathies-Present State, Challenges, and Perspectives.Molecules (Basel, Switzerland) · 2020Review
- Archiving and disseminating integrative structure models.Journal of biomolecular NMR · 2019Article
- Membrane protein structural biology in the era of single particle cryo-EM.Current opinion in structural biology · 2018Review
- The role of RyR2 oxidation in the blunted frequency-dependent facilitation of CaPflugers Archiv : European journal of physiology · 2018Article
- Integrative structure modeling with the Integrative Modeling Platform.Protein science : a publication of the Protein Society · 2018Article
- Redox Dependent Modifications of Ryanodine Receptor: Basic Mechanisms and Implications in Heart Diseases.Frontiers in physiology · 2018Review
- Cryo-electron tomography: an ideal method to study membrane-associated proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- CaTrends in biochemical sciences · 2017Review
- A guide to the 3D structure of the ryanodine receptor type 1 by cryoEM.Protein science : a publication of the Protein Society · 2017Review
- The N-Terminal Region of the Ryanodine Receptor Affects Channel Activation.Frontiers in physiology · 2017Article
- Calcium Channels in Vascular Smooth Muscle.Advances in pharmacology (San Diego, Calif.) · 2017Review
- De Novo modeling in cryo-EM density maps with Pathwalking.Journal of structural biology · 2016Article
- The Central domain of RyR1 is the transducer for long-range allosteric gating of channel opening.Cell research · 2016Article
- Structures of the colossal RyR1 calcium release channel.Current opinion in structural biology · 2016Review
- Single-particle electron microscopy in the study of membrane protein structure.Microscopy (Oxford, England) · 2016Article
- Increased Energy Demand during Adrenergic Receptor Stimulation Contributes to Ca(2+) Wave Generation.Biophysical journal · 2015Article
- Validation methods for low-resolution fitting of atomic structures to electron microscopy data.Archives of biochemistry and biophysics · 2015Article
- Membrane protein structures without crystals, by single particle electron cryomicroscopy.Current opinion in structural biology · 2015Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The skeletal muscle Ca(2+) release channel (RyR1), a homotetramer, regulates the release of Ca(2+) from the sarcoplasmic reticulum to initiate muscle contraction. In this work, we have delineated the RyR1 monomer boundaries in a subnanometer-resolution electron cryomicroscopy (cryo-EM) density map. In the cytoplasmic region of each RyR1 monomer, 36 alpha-helices and 7 beta-sheets can be resolved. A beta-sheet was also identified close to the membrane-spanning region that resembles the cytoplasmic pore structures of inward rectifier K(+) channels. Three structural folds, generated for amino acids 12-565 using comparative modeling and cryo-EM density fitting, localize close to regions implicated in communication with the voltage sensor in the transverse tubules. Eleven of the 15 disease-related residues for these domains are mapped to the surface of these models. Four disease-related residues are found in a basin at the interfaces of these regions, creating a pocket in which the immunophilin FKBP12 can fit. Taken together, these results provide a structural context for both channel gating and the consequences of certain malignant hyperthermia and central core disease-associated mutations in RyR1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.