ArticleProceedings of the National Academy of Sciences of the United States of America2008
Flavin-dependent thymidylate synthase X limits chromosomal DNA replication.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- tRNA pseudouridine synthase D (TruD) fromRNA (New York, N.Y.) · 2025Article
- Exploring microbial diversity and biosynthetic potential in zoo and wildlife animal microbiomes.Nature communications · 2024Article
- Functional Prokaryotic-Like Deoxycytidine Triphosphate Deaminases and Thymidylate Synthase in Eukaryotic Social Amoebae: Vertical, Endosymbiotic, or Horizontal Gene Transfer?Molecular biology and evolution · 2023Article
- Bacterial origins of thymidylate metabolism in Asgard archaea and Eukarya.Nature communications · 2023Article
- Genome Characterization of a Novel WastewaterFrontiers in microbiology · 2020Article
- Unique Features and Anti-microbial Targeting of Folate- and Flavin-Dependent Methyltransferases Required for Accurate Maintenance of Genetic Information.Frontiers in microbiology · 2018Review
- Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori.PloS one · 2018Article
- Article
- Conservation and Role of Electrostatics in Thymidylate Synthase.Scientific reports · 2015Article
- Constitutive Stringent Response Restores Viability of Bacillus subtilis Lacking Structural Maintenance of Chromosome Protein.PloS one · 2015Article
- Comparative genomics of nucleotide metabolism: a tour to the past of the three cellular domains of life.BMC genomics · 2014Article
- Epidemic Clostridium difficile strains demonstrate increased competitive fitness compared to nonepidemic isolates.Infection and immunity · 2014Article
- Article
- DprB facilitates inter- and intragenomic recombination in Helicobacter pylori.Journal of bacteriology · 2012Article
- Comparative (meta)genomic analysis and ecological profiling of human gut-specific bacteriophage φB124-14.PloS one · 2012Article
- Flavin-dependent thymidylate synthase: a novel pathway towards thymine.Archives of biochemistry and biophysics · 2010Article
- Evolutionary constraints permeate large metabolic networks.BMC evolutionary biology · 2009Article
- An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene.Nature · 2009Article
- Trends in prokaryotic evolution revealed by comparison of closely related bacterial and archaeal genomes.Journal of bacteriology · 2009Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the hitherto unexplored possibility that differences in the catalytic efficiencies of thymidylate synthases ThyX and ThyA, enzymes that produce the essential DNA precursor dTMP, have influenced prokaryotic genome evolution. We demonstrate that DNA replication speed in bacteria and archaea that contain the low-activity ThyX enzyme is up to 10-fold decreased compared with species that contain the catalytically more efficient ThyA. Our statistical studies of >400 genomes indicated that ThyA proteins are preferred for the replication of large genomes, providing further evidence that the thymidylate metabolism is limiting expansion of prokaryotic genomes. Because both ThyX and ThyA participate in frequent reciprocal gene replacement events, our observations indicate that the bacterial metabolism continues to modulate the size and composition of prokaryotic genomes. We also propose that the increased kinetic efficiency of thymidylate synthesis has contributed to extending the prokaryotic evolutionary potential.
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