ReviewAnalytica chimica acta2008
Mass spectrometry of the fifth nucleoside: a review of the identification of pseudouridine in nucleic acids.
Review in Analytica chimica acta, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- A combined pseudouridine biomanufacturing platform enabled by a streamlined designer pathway.Nature communications · 2025Article
- Temperature-Dependent tRNA Modifications in Bacillales.International journal of molecular sciences · 2024Article
- In-Gel Cyanoethylation for Pseudouridines Mass Spectrometry Detection of Bacterial Regulatory RNA.Methods in molecular biology (Clifton, N.J.) · 2024Article
- H/ACA snRNP-dependent ribosome biogenesis regulates translation of polyglutamine proteins.Science advances · 2023Article
- Data Analysis Pipeline for Detection and Quantification of Pseudouridine (ψ) in RNA by HydraPsiSeq.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Ribosomal RNA Pseudouridylation: Will Newly Available Methods Finally Define the Contribution of This Modification to Human Ribosome Plasticity?Frontiers in genetics · 2022Review
- Review
- The Dark Side of the Epitranscriptome: Chemical Modifications in Long Non-Coding RNAs.International journal of molecular sciences · 2017Review
- Detecting RNA modifications in the epitranscriptome: predict and validate.Nature reviews. Genetics · 2017Review
- Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QΨA) Is Catalyzed by the Dual Specificity Enzyme RluF.The Journal of biological chemistry · 2016Article
- Sequence mapping of transfer RNA chemical modifications by liquid chromatography tandem mass spectrometry.Methods (San Diego, Calif.) · 2016Article
- A mass spectrometry-based method for direct determination of pseudouridine in RNA.Nucleic acids research · 2016Article
- RoboOligo: software for mass spectrometry data to support manual and de novo sequencing of post-transcriptionally modified ribonucleic acids.Nucleic acids research · 2015Article
- The identification and characterization of non-coding and coding RNAs and their modified nucleosides by mass spectrometry.RNA biology · 2014Review
- Pseudouridine: still mysterious, but never a fake (uridine)!RNA biology · 2014Review
- A personal perspective on chemistry-driven RNA research.Biopolymers · 2013Article
- Mass spectrometry-based quantification of pseudouridine in RNA.Journal of the American Society for Mass Spectrometry · 2011Article
- Identification, localization, and relative quantitation of pseudouridine in RNA by tandem mass spectrometry of hydrolysis products.International journal of mass spectrometry · 2011Article
- Use of specific chemical reagents for detection of modified nucleotides in RNA.Journal of nucleic acids · 2011Article
- Hybrid activation methods for elucidating nucleic acid modifications.Analytical chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Pseudouridine, the so-called fifth nucleoside due to its ubiquitous presence in ribonucleic acids (RNAs), remains among the most challenging modified nucleosides to characterize. As an isomer of the major nucleoside uridine, pseudouridine cannot be detected by standard reverse-transcriptase-based DNA sequencing or RNase mapping approaches. Thus, over the past 15 years, investigators have focused on the unique structural properties of pseudouridine to develop selective derivatization or fragmentation strategies for its determination. While the N-cyclohexyl-N'-beta-(4-methylmorpholinium)ethylcarbodiimide p-tosylate (CMCT)-reverse transcriptase assay remains both a popular and powerful approach to screen for pseudouridine in larger RNAs, mass-spectrometry-based approaches are poised to play an increasingly important role in either confirming the findings of the CMCT-reverse transcriptase assay or in characterizing pseudouridine sequence placement and abundance in smaller RNAs. This review includes a brief discussion of pseudouridine including a summary of its biosynthesis and known importance within various RNAs. The review then focuses on chemical derivatization approaches that can be used to selectively modify pseudouridine to improve its detection, and the development of mass-spectrometry-based assays for the identification and sequencing of pseudouridine in various RNAs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.