ArticleProceedings of the National Academy of Sciences of the United States of America1991
Preferential integration of marker DNA into the chromosomal fragile site at 3p14: an approach to cloning fragile sites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 109 citations in OpenAlex.
- High-efficiency and multilocus targeted integration in CHO cells using CRISPR-mediated donor nicking and DNA repair inhibitors.Biotechnology and bioengineering · 2023Article
- The Impact of 9-Valent HPV Vaccination on Couple Infertility Prevention: A Comprehensive Review.Frontiers in medicine · 2021Review
- Fragile sites in cancer: more than meets the eye.Nature reviews. Cancer · 2017Review
- MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering.Biotechnology and bioengineering · 2017Article
- Molecular characterization of common fragile sites as a strategy to discover cancer susceptibility genes.Cellular and molecular life sciences : CMLS · 2014Review
- Recombinogenic conditions influence partner choice in spontaneous mitotic recombination.PLoS genetics · 2013Article
- Chromosome breakages associated with 45S ribosomal DNA sequences in spotted snakehead fish Channa punctatus.Molecular biology reports · 2013Article
- Infection with retroviral vectors leads to perturbed DNA replication increasing vector integrations into fragile sites.Scientific reports · 2013Article
- Article
- Common fragile site tumor suppressor genes and corresponding mouse models of cancer.Journal of biomedicine & biotechnology · 2011Review
- Integration of exogenous DNA into mouse embryonic stem cell chromosomes shows preference into genes and frequent modification at junctions.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Fhit tumor suppressor: guardian of the preneoplastic genome.Future oncology (London, England) · 2008Review
- Article
- Replication stress induces tumor-like microdeletions in FHIT/FRA3B.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Human papilloma virus DNA exposure and embryo survival is stage-specific.Journal of assisted reproduction and genetics · 2006Article
- Premature condensation induces breaks at the interface of early and late replicating chromosome bands bearing common fragile sites.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Large-scale analysis of adeno-associated virus vector integration sites in normal human cells.Journal of virology · 2005Article
- Chromosomal instability at common fragile sites in Seckel syndrome.American journal of human genetics · 2004Article
- BRCA1 is required for common-fragile-site stability via its G2/M checkpoint function.Molecular and cellular biology · 2004Article
- Fragile site orthologs FHIT/FRA3B and Fhit/Fra14A2: evolutionarily conserved but highly recombinogenic.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Fragile sites are specific regions of chromosomes that are prone to breakage. In cells cultured under conditions that induce fragile site expression, high levels of inter- and intrachromosomal recombination have been observed involving chromosomal bands containing fragile sites. To determine whether expression of specific fragile sites would facilitate preferential integration of exogenous DNA at these recombination hot spots, the vector pSV2Neo was transfected into a Chinese hamster-human somatic cell hybrid containing a derivative chromosome 3 as its only human component. Chromosome 3 contains a common fragile site at band 3p14.2 (FRA3B) that is induced by aphidicolin. Both cells induced to express FRA3B and the uninduced control cells were transfected with the pSV2Neo selectable plasmid. In situ hybridization of a biotin-labeled pSV2Neo probe to metaphase chromosomes revealed one to three integration sites in each stably transfected clone. Four of 13 clones transfected under conditions of FRA3B induction showed integration of pSV2Neo at 3p14; these clones also showed specific integration into hamster chromosome 1 and a rearranged chromosome characteristic of CHO cells (mar2). The 7 control clones, however, showed an apparently random pattern of pSV2Neo integration. Significant hybridization of pSV2Neo to both FRA3B and Chinese hamster chromosomes 1 and mar2 was seen in 100 cells from pooled colonies transfected after treatment with aphidicolin. These results suggest that preferential integration of marker DNA into human and Chinese hamster fragile sites occurs with exposure to aphidicolin. The nature of the DNA sequences at fragile sites is unknown and, despite a number of approaches, these sequences have not yet been isolated; our procedure may represent an approach to the cloning of fragile sites.
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