ArticleProceedings of the National Academy of Sciences of the United States of America1991
Cholinesterase-like domains in enzymes and structural proteins: functional and evolutionary relationships and identification of a catalytically essential aspartic acid.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 147 citations in OpenAlex.
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- Thyroglobulin Represents a Novel Molecular Architecture of Vertebrates.The Journal of biological chemistry · 2016Article
- Neuromuscular Junction Impairment in Amyotrophic Lateral Sclerosis: Reassessing the Role of Acetylcholinesterase.Frontiers in molecular neuroscience · 2016Review
- Tracking the origin and divergence of cholinesterases and neuroligins: the evolution of synaptic proteins.Journal of molecular neuroscience : MN · 2014Article
- Acetylcholinesterase protein level is preserved in the Alzheimer's brain.Journal of molecular neuroscience : MN · 2014Article
- Phylogenetic analysis of the human thyroglobulin regions.Thyroid research · 2012Article
- Article
- Alicyclobacillus acidocaldarius thermophilic esterase EST2's activity in milk and cheese models.Applied and environmental microbiology · 2006Article
- Investigation of the catalytic triad of arylamine N-acetyltransferases: essential residues required for acetyl transfer to arylamines.The Biochemical journal · 2005Article
- Acetylcholinesterase promotes neurite elongation, synapse formation, and surface expression of AMPA receptors in hippocampal neurones.Molecular and cellular neurosciences · 2003Article
- Neuropathy target esterase.The Biochemical journal · 1999Review
- Acetylcholinesterase of Schistosoma mansoni--functional correlates. Contributed in honor of Professor Hans Neurath's 90th birthday.Protein science : a publication of the Protein Society · 1999Review
- Homology modeling and active-site residues probing of the thermophilic Alicyclobacillus acidocaldarius esterase 2.Protein science : a publication of the Protein Society · 1999Article
- Acetylcholinesterase gene expression in axotomized rat facial motoneurons is differentially regulated by neurotrophins: correlation with trkB and trkC mRNA levels and isoforms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Overexpression and properties of a new thermophilic and thermostable esterase from Bacillus acidocaldarius with sequence similarity to hormone-sensitive lipase subfamily.The Biochemical journal · 1998Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primary sequences of cholinesterases and related proteins have been systematically compared. The cholinesterase-like domain of these proteins, about 500 amino acids, may fulfill a catalytic and a structural function. We identified an aspartic acid residue that is conserved among esterases and lipases (Asp-397 in Torpedo acetylcholinesterase) but that had not been considered to be involved in the catalytic mechanism. Site-directed mutagenesis demonstrated that this residue is necessary for activity. Analysis of evolutionary relationships shows that the noncatalytic members of the family do not constitute a separate subgroup, suggesting that loss of catalytic activity occurred independently on several occasions, probably from bifunctional molecules. Cholinesterases may thus be involved in cell-cell interactions in addition to the hydrolysis of acetylcholine. This would explain their specific expression in well-defined territories during embryogenesis before the formation of cholinergic synapses and their presence in noncholinergic tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.