ArticleReproductive biology and endocrinology : RB&E2008
Gene silencing by RNAi in mouse Sertoli cells.
Article in Reproductive biology and endocrinology : RB&E, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice.Journal of assisted reproduction and genetics · 2024Article
- RNA Interference as a Method of Gene Knockdown in Cultured Spermatogonia.Methods in molecular biology (Clifton, N.J.) · 2023Article
- MicroRNA-322 Regulates Self-renewal of Mouse Spermatogonial Stem Cells throughInternational journal of biological sciences · 2019Article
- Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis.Cell reports · 2017Article
- MicroRNA-224 regulates self-renewal of mouse spermatogonial stem cells via targeting DMRT1.Journal of cellular and molecular medicine · 2016Article
- Chemokine (C-X-C) Ligand 12 Facilitates Trafficking of Donor Spermatogonial Stem Cells.Stem cells international · 2016Article
- RNAi-mediated knockdown of INHBB increases apoptosis and inhibits steroidogenesis in mouse granulosa cells.The Journal of reproduction and development · 2015Article
- In vivo microinjection and electroporation of mouse testis.Journal of visualized experiments : JoVE · 2014Article
- Integrating microRNAs into the complexity of gonadotropin signaling networks.Frontiers in cell and developmental biology · 2013Review
- Lentiviral transduction of rat Sertoli cells as a means to modify gene expression.Spermatogenesis · 2012Article
- MicroRNA-21 regulates the self-renewal of mouse spermatogonial stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA interference (RNAi) is a valuable tool in the investigation of gene function. The purpose of this study was to examine the availability, target cell types and efficiency of RNAi in the mouse seminiferous epithelium.
methodsThe experimental model was based on transgenic mice expressing EGFP (enhanced green fluorescent protein). RNAi was induced by in vivo transfection of plasmid vectors encoding for short hairpin RNAs (shRNAs) targeting EGFP. shRNAs were transfected in vivo by microinjection into the seminiferous tubules via the rete testis followed by square wave electroporation. As a transfection reporter, expression of red fluorescent protein (HcRed 1) was used. Cell types, the efficiency of both transfections and RNAi were all evaluated.
resultsSertoli cells were the main transfected cells. A reduction of about 40% in the level of EGFP protein was detected in cells successfully transfected both in vivo and in vitro. However, the efficiency of in vivo transfection was low.
conclusionIn adult seminiferous epithelial cells, in vivo post-transcriptional gene silencing mediated by RNAi via shRNA is efficient in Sertoli cells. Similar levels of RNAi were detected both in vivo and in vitro. This also indicates that Sertoli cells have the necessary silencing machinery to repress the expression of endogenous genes via RNAi.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.