ArticleBMC genomics2008
The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 194 citations in OpenAlex.
- Safety evaluation of the food enzyme cellulase from the genetically modifiedEFSA journal. European Food Safety Authority · 2026Article
- Safety evaluation of the food enzyme triacylglycerol lipase from the genetically modifiedEFSA journal. European Food Safety Authority · 2026Article
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- Safety evaluation of the food enzyme endo-polygalacturonase from the genetically modifiedEFSA journal. European Food Safety Authority · 2025Article
- Safety evaluation of the food enzyme β-fructofuranosidase from the genetically modifiedEFSA journal. European Food Safety Authority · 2025Article
- Safety evaluation of the food enzyme fructan β-fructosidase from the genetically modifiedEFSA journal. European Food Safety Authority · 2024Article
- Genomic Characterization and Establishment of a Genetic Manipulation System forJournal of fungi (Basel, Switzerland) · 2024Article
- The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei.Communications biology · 2024Article
- Effect of the res2 transcription factor gene deletion on protein secretion and stress response in the hyperproducer strain Trichoderma reesei Rut-C30.BMC microbiology · 2023Article
- Safety evaluation of the food enzyme pectin lyase from the genetically modifiedEFSA journal. European Food Safety Authority · 2022Article
- Safety evaluation of the food enzyme mannan endo-1,4-β-mannosidase from the genetically modifiedEFSA journal. European Food Safety Authority · 2022Article
- Safety evaluation of the food enzyme glucose oxidase from the genetically modifiedEFSA journal. European Food Safety Authority · 2022Article
- Integrative transcriptome and proteome analyses of Trichoderma longibrachiatum LC and its cellulase hyper-producing mutants generated by heavy ion mutagenesis reveal the key genes involved in cellulolytic enzymes regulation.Biotechnology for biofuels and bioproducts · 2022Article
- Factors regulating cellulolytic gene expression in filamentous fungi: an overview.Microbial cell factories · 2022Review
- Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2022Article
- Review
- Current perspective on production and applications of microbial cellulases: a review.Bioresources and bioprocessing · 2021Review
- Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei.Scientific reports · 2021Article
- Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.BMC genomics · 2020Article
- Safety evaluation of the food enzyme Phospholipase AEFSA journal. European Food Safety Authority · 2020Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe hypercellulolytic mutant Hypocrea jecorina (anamorph Trichoderma reesei) RUT C30 is the H. jecorina strain most frequently used for cellulase fermentations and has also often been employed for basic research on cellulase regulation. This strain has been reported to contain a truncated carbon catabolite repressor gene cre1 and is consequently carbon catabolite derepressed. To date this and an additional frame-shift mutation in the glycoprotein-processing beta-glucosidase II encoding gene are the only known genetic differences in strain RUT C30.
resultsIn the present paper we show that H. jecorina RUT C30 lacks an 85 kb genomic fragment, and consequently misses additional 29 genes comprising transcription factors, enzymes of the primary metabolism and transport proteins. This loss is already present in the ancestor of RUT C30--NG 14--and seems to have occurred in a palindromic AT-rich repeat (PATRR) typically inducing chromosomal translocations, and is not linked to the cre1 locus. The mutation of the cre1 locus has specifically occurred in RUT C30. Some of the genes that are lacking in RUT C30 could be correlated with pronounced alterations in its phenotype, such as poor growth on alpha-linked oligo- and polyglucosides (loss of maltose permease), or disturbance of osmotic homeostasis.
conclusionOur data place a general caveat on the use of H. jecorina RUT C30 for further basic research.
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