ArticleInternational journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases2008
Asparaginyl endopeptidase from the carcinogenic liver fluke, Opisthorchis viverrini, and its potential for serodiagnosis.
Article in International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Enhanced serodiagnosis of opisthorchiasis using a multi-epitope dot-ELISA: comparative evaluation of visual and imageJ-assisted analysis of IgG and IgM responses.BMC infectious diseases · 2026Article
- Current Prevalence of Infection with Opisthorchis viverrini and Intestinal Parasites in Rural Communities in Northeast Thailand Using Stool Examinations and Serological Screening Methods.The American journal of tropical medicine and hygiene · 2025Article
- Multi-epitope protein production and its application in the diagnosis of opisthorchiasis.Parasites & vectors · 2024Article
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- Production and Immunological Characterization of scFv Specific to Epitope ofTropical medicine and infectious disease · 2023Article
- Epidemiology and Control of Opisthorchis viverrini Infection: Implications for Cholangiocarcinoma Prevention.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2023Article
- An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis ofTropical medicine and infectious disease · 2022Article
- Comparative assessment of immunochromatographic test kits using somatic antigens from adult Opisthorchis viverrini and IgG and IgG4 conjugates for serodiagnosis of human opisthorchiasis.Parasitology research · 2021Article
- Development of an Immunochromatographic Point-of-Care Test for Serodiagnosis of Opisthorchiasis and Clonorchiasis.The American journal of tropical medicine and hygiene · 2019Article
- Opisthorchis viverrini Proteome and Host-Parasite Interactions.Advances in parasitology · 2018Review
- Immunodiagnosis of opisthorchiasis using parasite cathepsin F.Parasitology research · 2015Article
- Article
- Identification and characterization of an asparaginyl endopeptidase from Angiostrongylus cantonensis.Parasitology research · 2014Article
- Evaluation of liver fluke recombinant cathepsin B-1 protease as a serodiagnostic antigen for human opisthorchiasis.Parasitology international · 2012Article
- Molecular changes in Opisthorchis viverrini (Southeast Asian liver fluke) during the transition from the juvenile to the adult stage.PLoS neglected tropical diseases · 2012Article
- Opisthorchiasis and Opisthorchis-associated cholangiocarcinoma in Thailand and Laos.Acta tropica · 2011Review
- Detection of salivary antibodies to crude antigens of Opisthorchis viverrini in opisthorchiasis and cholangiocarcinoma patients.Clinical oral investigations · 2011Article
- The secreted and surface proteomes of the adult stage of the carcinogenic human liver fluke Opisthorchis viverrini.Proteomics · 2010Article
- Ov-APR-1, an aspartic protease from the carcinogenic liver fluke, Opisthorchis viverrini: functional expression, immunolocalization and subsite specificity.The international journal of biochemistry & cell biology · 2009Article
- Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini.PLoS neglected tropical diseases · 2009Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
objectivesTo isolate and characterize an asparaginyl endopeptidase from the carcinogenic liver fluke, Opisthorchis viverrini, and evaluate its expression profile, biochemical activity, and potential as an immunodiagnostic antigen.
methodsThe full length mRNA encoding an asparaginyl endopeptidase (family C13), Ov-aep-1, was isolated by immunoscreening of a cDNA bacteriophage library of adult O. viverrini using sera from patients infected with O. viverrini. Investigation of Ov-aep-1 transcripts in developmental stages of the parasite, and phylogenetic analysis, immunohistochemical localization, and recombinant protein expression and enzymology were employed to characterize the Ov-AEP-1 protein. Immunoblotting was used to assess the potential of this enzyme for immunodiagnosis of human opisthorchiasis.
resultsOv-AEP-1 is characteristic of the C13 cysteine protease family. Ov-aep-1 transcripts were detected in adult and juvenile worms, eggs, and metacercariae. Phylogenetic analysis indicated that Ov-AEP-1 is closely related to homologous proteins in other trematodes. Recombinant Ov-AEP-1 was expressed in bacteria in inclusion bodies and refolded to a soluble form. Excretory-secretory (ES) products derived from adult O. viverrini and refolded recombinant Ov-AEP-1 both displayed catalytic activity against the diagnostic tripeptide substrate, Ala-Ala-Asn-aminomethylcoumarin. Rabbit antiserum raised to recombinant Ov-AEP-1 identified the native AEP-1 protease in both somatic extract and ES products of adult worms. Anti-Ov-AEP-1 IgG immunolocalized the anatomical site of expression to the gut of the fluke, implying a physiological role in digestion of food or activation of other digestive enzymes. Recombinant Ov-AEP-1 was recognized by serum antibodies from patients with opisthorchiasis but not other helminth infections, with a sensitivity and specificity of 85% and 100%, respectively. The positive and negative predictive values are 100% and 67%, respectively.
conclusionsThe liver fluke, O. viverrini, has a gut-localized asparaginyl endopeptidase. Refolded recombinant Ov-AEP-1 is catalytically active and has potential for immunodiagnosis of human opisthorchiasis.
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