ArticleCurrent biology : CB2008
Osmotic regulation of Rab-mediated organelle docking.
Article in Current biology : CB, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Hydration gradients drive lipid self-segregation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The GTPase activating protein Gyp7 regulates Rab7/Ypt7 activity on late endosomes.The Journal of cell biology · 2024Article
- A two-tiered system for selective receptor and transporter protein degradation.PLoS genetics · 2022Article
- Acetate and hypertonic stress stimulate vacuole membrane fission using distinct mechanisms.PloS one · 2022Article
- A kinase cascade on the yeast lysosomal vacuole regulates its membrane dynamics: conserved kinase Env7 is phosphorylated by casein kinase Yck3.The Journal of biological chemistry · 2020Article
- The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation.Nature communications · 2018Article
- SNARE-mediated membrane fusion arrests at pore expansion to regulate the volume of an organelle.The EMBO journal · 2018Article
- The NaMolecular biology of the cell · 2018Article
- How and why intralumenal membrane fragments form during vacuolar lysosome fusion.Molecular biology of the cell · 2017Article
- A HOPS Protein, MoVps41, Is Crucially Important for Vacuolar Morphogenesis, Vegetative Growth, Reproduction and Virulence inFrontiers in plant science · 2017Article
- Adjustment of host cells for accommodation of symbiotic bacteria: vacuole defunctionalization, HOPS suppression, and TIP1g retargeting in Medicago.The Plant cell · 2014Article
- Tracking of the dynamic localization of the Rab-specific HOPS subunits reveal their distinct interaction with Ypt7 and vacuoles.Cellular logistics · 2014Article
- Characterization of an M28 metalloprotease family member residing in the yeast vacuole.FEMS yeast research · 2013Article
- Bioanalysis of eukaryotic organelles.Chemical reviews · 2013Review
- Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole.Molecular and cellular biology · 2013Article
- Yeast vacuoles fragment in an asymmetrical two-phase process with distinct protein requirements.Molecular biology of the cell · 2012Article
- Phosphorylation of a membrane curvature-sensing motif switches function of the HOPS subunit Vps41 in membrane tethering.The Journal of cell biology · 2010Article
- Vps-C complexes: gatekeepers of endolysosomal traffic.Current opinion in cell biology · 2009Review
- Capture and release of partially zipped trans-SNARE complexes on intact organelles.The Journal of cell biology · 2009Article
- Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase.The Journal of cell biology · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Osmotic gradients across organelle and plasma membranes modulate the rates of membrane fission and fusion; sufficiently large gradients can cause membrane rupture [1-6]. Hypotonic gradients applied to living yeast cells trigger prompt (within seconds) swelling and fusion of Saccharomyces cerevisiae vacuoles, whereas hypertonic gradients cause vacuoles to fragment on a slower time scale [7-11]. Here, we analyze the influence of osmotic strength on homotypic fusion of isolated yeast vacuoles. Consistent with previously reported in vivo results, we find that decreases in osmolyte concentration increase the rate and extent of vacuole fusion in vitro, whereas increases in osmolyte concentration prevent fusion. Unexpectedly, our results reveal that osmolytes regulate fusion by inhibiting early Rab-dependent docking or predocking events, not late events. Our experiments reveal an organelle-autonomous pathway that may control organelle surface-to-volume ratio, size, and copy number: Decreasing the osmolyte concentration in the cytoplasmic compartment accelerates Rab-mediated docking and fusion. By altering the relationship between the organelle surface and its enclosed volume, fusion in turn reduces the risk of membrane rupture.
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