ArticleNucleic acids research1991
Nucleotide preferences in sequence-specific recognition of DNA by c-myb protein.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 108 citations in OpenAlex.
- The White Clover TrMYB33-TrSAMS1 Module Contributes to Drought Tolerance by Modulation of Spermidine Biosynthesis via an ABA-Dependent Pathway.International journal of molecular sciences · 2024Article
- MYB: A Key Transcription Factor in the Hematopoietic System Subject to Many Levels of Control.Advances in experimental medicine and biology · 2024Review
- Expression dynamics of metalloproteinases during mandibular bone formation: association with Myb transcription factor.Frontiers in cell and developmental biology · 2023Article
- Insights into the Diversification and Evolution of R2R3-MYB Transcription Factors in Plants.Plant physiology · 2020Article
- Reading More than Histones: The Prevalence of Nucleic Acid Binding among Reader Domains.Molecules (Basel, Switzerland) · 2018Review
- MYB - A regulatory factor in hematopoiesis.Gene · 2018Review
- Polymorphism within a Neuronal Activity-Dependent Enhancer of NgR1 Is Associated with Corpus Callosum Morphology in Humans.Molecular neuropsychiatry · 2015Article
- The conserved transcription factors, MYB115 and MYB118, control expression of the newly evolved benzoyloxy glucosinolate pathway in Arabidopsis thaliana.Frontiers in plant science · 2015Article
- Tight interconnection and multi-level control of Arabidopsis MYB44 in MAPK cascade signalling.PloS one · 2013Article
- Quadruple 9-mer-based protein binding microarray analysis confirms AACnG as the consensus nucleotide sequence sufficient for the specific binding of AtMYB44.Molecules and cells · 2012Article
- Cell fate in the Arabidopsis root epidermis is determined by competition between WEREWOLF and CAPRICE.Plant physiology · 2011Article
- A noncoding point mutation of Zeb1 causes multiple developmental malformations and obesity in Twirler mice.PLoS genetics · 2011Article
- B-MYB delays cell aging by repressing p16 (INK4α) transcription.Cellular and molecular life sciences : CMLS · 2011Article
- GFI1B controls its own expression binding to multiple sites.Haematologica · 2010Article
- Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes.Plant physiology · 2009Article
- A Lin-9 complex is recruited by B-Myb to activate transcription of G2/M genes in undifferentiated embryonal carcinoma cells.Oncogene · 2009Article
- Article
- Atomic model of human Rcd-1 reveals an armadillo-like-repeat protein with in vitro nucleic acid binding properties.Protein science : a publication of the Protein Society · 2007Article
- Article
- E2Fs link the control of G1/S and G2/M transcription.The EMBO journal · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a binding site selection procedure, we have found that sequence-specific DNA-binding by the mouse c-myb protein involves recognition of nucleotides outside of the previously identified hexanucleotide motif. Oligonucleotides containing a random nucleotide core were immunoprecipitated in association with c-Myb, amplified by the Polymerase Chain Reaction and cloned in plasmids prior to sequencing. By alignment of sequences it was apparent that additional preferences existed at each of three bases immediately 5' of the hexanucleotide consensus, allowing an extension of the preferred binding site to YGRCVGTTR. The contributions of these 5' nucleotides to binding affinity was established in bandshift analyses with oligonucleotides containing single base substitutions; in particular, it was found that replacement of the preferred guanine at position -2 with any other base greatly reduced c-Myb binding. We found that the protein encoded by the related B-myb gene bound the preferred c-Myb site with similar affinity; however, B-Myb and c-Myb showed distinct preferences for the identity of the nucleotide at position -1 relative to the hexanucleotide consensus. This study demonstrates that the c-Myb DNA-binding site is more extensive than recognised hitherto and points to similar but distinct nucleotide preferences in recognition of DNA by related Myb proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.