ArticleHistochemistry and cell biology2008
Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney.
Article in Histochemistry and cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 67 citations in OpenAlex.
- Disruption of mitochondrial electron transport impairs urinary concentration via AMPK-dependent suppression of aquaporin 2.JCI insight · 2024Article
- Highly parallel production of designer organoids by mosaic patterning of progenitors.Cell systems · 2024Article
- Endogenous renal adiponectin drives gluconeogenesis through enhancing pyruvate and fatty acid utilization.Nature communications · 2023Article
- Discrete localization of phospholipase Cβ3 and diacylglycerol kinase ι along the renal proximal tubules of normal rat kidney and gentamicin-induced changes in their expression.Histochemistry and cell biology · 2023Article
- A comparative study of cellular diversity between the Xenopus pronephric and mouse metanephric nephron.Kidney international · 2023Article
- An organ-derived extracellular matrix triggers in situ kidney regeneration in a preclinical model.NPJ Regenerative medicine · 2022Article
- Connecting tubules develop from the tip of the ureteric bud in the human kidney.Histochemistry and cell biology · 2021Article
- How to distinguish between different cell lineages sharing common markers using combinations of double in-situ-hybridization and immunostaining in avian embryos: CXCR4-positive mesodermal and neural crest-derived cells.Histochemistry and cell biology · 2021Article
- Single-Cell Profiling of AKI in a Murine Model Reveals Novel Transcriptional Signatures, Profibrotic Phenotype, and Epithelial-to-Stromal Crosstalk.Journal of the American Society of Nephrology : JASN · 2020Article
- Advances in understanding vertebrate nephrogenesis.Tissue barriers · 2020Article
- Differentiating Induced Pluripotent Stem Cells into Renal Cells: A New Approach to Treat Kidney Diseases.Stem cells international · 2020Review
- Kidney Regeneration in Later-Stage Mouse EmbryosJournal of the American Society of Nephrology : JASN · 2019Article
- HPD degradation regulated by the TTC36-STK33-PELI1 signaling axis induces tyrosinemia and neurological damage.Nature communications · 2019Article
- Article
- Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells.Stem cells international · 2017Article
- Nephron organoids derived from human pluripotent stem cells model kidney development and injury.Nature biotechnology · 2015Article
- Review
- Directed Differentiation of Pluripotent Stem Cells into Kidney.Biomarker insights · 2015Review
- Induction and patterning of the metanephric nephron.Seminars in cell & developmental biology · 2014Review
- Single cell dissection of early kidney development: multilineage priming.Development (Cambridge, England) · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The kidney is the most complex organ within the urogenital system. The adult mouse kidney contains in excess of 8,000 mature nephrons, each of which can be subdivided into a renal corpuscle and 14 distinct tubular segments. The histological complexity of this organ can make the clarification of the site of gene expression by in situ hybridisation difficult. We have defined a panel of seven antibodies capable of identifying the six stages of early nephron development, the tubular nephron segments and the components of the renal corpuscle within the embryonic and adult mouse kidney. We have analysed in detail the protein expression of Wt1, Calb1 Aqp1, Aqp2 and Umod using these antibodies. We have then coupled immunohistochemistry with RNA in situ hybridisation in order to precisely identify the expression pattern of different genes, including Wnt4, Umod and Spp1. This technique will be invaluable for examining at high resolution, the structure of both the developing and mature nephron where standard in situ hybridisation and histological techniques are insufficient. The use of this technique will enhance the expression analyses of genes which may be involved in nephron formation and the function of the mature nephron in the mouse.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.