ArticlePLoS neglected tropical diseases2008
A single-step sequencing method for the identification of Mycobacterium tuberculosis complex species.
Article in PLoS neglected tropical diseases, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A new diagnostic kit using real-time polymerase chain reaction for the detection of Mycobacterium tuberculosis.AMB Express · 2026Article
- First report of fatal feline pulmonary tuberculosis caused by the emerging zoonotic pathogen Mycobacterium orygis in a cat from India.Veterinary research communications · 2026Article
- Correction: A Single-Step Sequencing Method for the Identification of Mycobacterium tuberculosis Complex Species.PLoS neglected tropical diseases · 2024Article
- Expression of Concern: A Single-Step Sequencing Method for the Identification of Mycobacterium tuberculosis Complex Species.PLoS neglected tropical diseases · 2022Article
- Mycobacterium bovis Pulmonary Tuberculosis, Algeria.Emerging infectious diseases · 2021Article
- Article
- Accurate Identification of Closely RelatedFrontiers in cellular and infection microbiology · 2021Article
- Multispacer Sequence Typing forFrontiers in veterinary science · 2021Article
- Emergence and clonal transmission of multi-drug-resistant tuberculosis among patients in Chad.BMC infectious diseases · 2017Article
- Innate defects of the IL-12/IFN-γ axis in susceptibility to infections by mycobacteria and salmonella.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2014Review
- Standing of nucleic acid testing strategies in veterinary diagnosis laboratories to uncover Mycobacterium tuberculosis complex members.Frontiers in molecular biosciences · 2014Review
- Importance of the genetic diversity within the Mycobacterium tuberculosis complex for the development of novel antibiotics and diagnostic tests of drug resistance.Antimicrobial agents and chemotherapy · 2012Review
- Diagnosis of latent tuberculosis infection in healthy young adults in a country with high tuberculosis burden and BCG vaccination at birth.BMC research notes · 2012Article
- SeekTB, a two-stage multiplex real-time-PCR-based method for differentiation of the Mycobacterium tuberculosis complex.Journal of clinical microbiology · 2012Article
- Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based single nucleotide polymorphism genotyping assay using iPLEX gold technology for identification of Mycobacterium tuberculosis complex species and lineages.Journal of clinical microbiology · 2011Article
- A novel multiplex real-time PCR for the identification of mycobacteria associated with zoonotic tuberculosis.PloS one · 2011Article
- Article
- Rapid identification of mycobacterial whole cells in solid and liquid culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2010Article
- Identification and genotyping of Mycobacterium tuberculosis complex species by use of a SNaPshot Minisequencing-based assay.Journal of clinical microbiology · 2010Article
- First insight into genetic diversity of the Mycobacterium tuberculosis complex in Albania obtained by multilocus variable-number tandem-repeat analysis and spoligotyping reveals the presence of beijing multidrug-resistant isolates.Journal of clinical microbiology · 2009Article
Corrections and comments
- Expression of concern · 2022-12-13Investigation by Journal/Publisher ·
- Erratum issued
- Expression of concern
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Mycobacterium tuberculosis complex (MTC) comprises closely related species responsible for strictly human and zoonotic tuberculosis. Accurate species determination is useful for the identification of outbreaks and epidemiological links. Mycobacterium africanum and Mycobacterium canettii are typically restricted to Africa and M. bovis is a re-emerging pathogen. Identification of these species is difficult and expensive. METHODOLOGY/PRINCIPAL
findingsThe Exact Tandem Repeat D (ETR-D; alias Mycobacterial Interspersed Repetitive Unit 4) was sequenced in MTC species type strains and 110 clinical isolates, in parallel to reference polyphasic identification based on phenotype profiling and sequencing of pncA, oxyR, hsp65, gyrB genes and the major polymorphism tandem repeat. Inclusion of M. tuberculosis isolates in the expanding, antibiotic-resistant Beijing clone was determined by Rv0927c gene sequencing. The ETR-D (780-bp) sequence unambiguously identified MTC species type strain except M. pinnipedii and M. microti thanks to six single nucleotide polymorphisms, variable numbers (1-7 copies) of the tandem repeat and two deletions/insertions. The ETR-D sequencing agreed with phenotypic identification in 107/110 clinical isolates and with reference polyphasic molecular identification in all isolates, comprising 98 M. tuberculosis, 5 M. bovis BCG type, 5 M. canettii, and 2 M. africanum. For M. tuberculosis isolates, the ETR-D sequence was not significantly associated with the Beijing clone. CONCLUSIONS/SIGNIFICANCE: ETR-D sequencing allowed accurate, single-step identification of the MTC at the species level. It circumvented the current expensive, time-consuming polyphasic approach. It could be used to depict epidemiology of zoonotic and human tuberculosis, especially in African countries where several MTC species are emerging.
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