ArticleAmerican journal of respiratory cell and molecular biology2009
Identification of a cigarette smoke-responsive region in the distal MMP-1 promoter.
Article in American journal of respiratory cell and molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 2 of them syntheses that pooled it.
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Who cites it
47 citing papers in PubMed, 2 syntheses or guidelines pooled it, 77 citations in OpenAlex.
- Biomarkers Identification in the Microenvironment of Oral Squamous Cell Carcinoma: A Systematic Review of Proteomic Studies.International journal of molecular sciences · 2024Pooled it
- Genetic polymorphism of matrix metalloproteinase family and chronic obstructive pulmonary disease susceptibility: a meta-analysis.Scientific reports · 2013Pooled it
- Cigarette smoke induces MUC5AC protein expression through the activation of Sp1.The Journal of biological chemistry · 2012Trial
- Molecular Links Between Smoking, COPD, and Lung Cancer: A DNA Methylation Perspective.Cancers · 2026Article
- Article
- Positive association between serum resistin and smoking was strongest in homozygotes of the G-A haplotype at c.-420 C>G and c.-358 G>A in RETN promoter: the Toon Genome Study.Journal of human genetics · 2023Article
- DNA Methylation of the Dopamine TransporterInternational journal of molecular sciences · 2023Article
- Article
- Methylation in the Promoter Region of the Dopamine TransporterInternational journal of environmental research and public health · 2022Article
- PEDF, a pleiotropic WTC-LI biomarker: Machine learning biomarker identification and validation.PLoS computational biology · 2021Article
- Suppression of cigarette smoke induced MMP1 expression by selective serotonin re-uptake inhibitors.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- A disintegrin and metalloproteinase domain-15 deficiency leads to exaggerated cigarette smoke-induced chronic obstructive pulmonary disease (COPD)-like disease in mice.Mucosal immunology · 2021Article
- Phloretin, an Apple Polyphenol, Inhibits Pathogen-Induced Mucin Overproduction.Molecular nutrition & food research · 2021Article
- Multi-endpoint analysis of human 3D airway epithelium following repeated exposure to whole electronic vapor product aerosol or cigarette smoke.Current research in toxicology · 2021Article
- Value of metalloproteinases in predicting COPD in heavy urban smokers.Respiratory research · 2020Observational
- Early effects of cigarette smoke extract on human oral keratinocytes and carcinogenesis in head and neck squamous cell carcinoma.Head & neck · 2020Article
- ADAM15 expression is increased in lung CD8Respiratory research · 2020Article
- Nicotine attenuates global genomic DNA methylation by influencing DNMTs gene expression in human endometrial stromal cells.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2020Article
- Validated inference of smoking habits from blood with a finite DNA methylation marker set.European journal of epidemiology · 2019Article
- Matrix Metalloproteinases in COPD and atherosclerosis with emphasis on the effects of smoking.PloS one · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Tobacco-related diseases are leading causes of death worldwide, and many are associated with expression of matrix metalloproteinase-1 (MMP-1). We have reported extracellular signal-regulated kinase (ERK)1/2-dependent induction of MMP-1 by cigarette smoke in lung epithelial cells. Our objectives were to define regions of the human MMP-1 promoter required for activation by smoke, to identify differences in responses of the 1G/2G -1607 polymorphic promoters to smoke, and to identify relevant transcription factors whose activity in airway epithelial cells is increased by smoke. The responses of deletion and mutant promoter constructs were measured in transfected cells during exposure to cigarette smoke extract (CSE). DNA oligonucleotide arrays were used to identify transcription factors activated after smoke exposure. CSE activated the MMP-1 promoter, and this induction was prevented by PD98059 blockade of ERK1/2 phosphorylation. Deletion studies revealed the distal 1kb promoter region (-4438 to -3280 upstream of the transcription start site) is essential for CSE induction of MMP-1, and confers activation of a minimal promoter. Studies of 1G and 2G MMP-1 polymorphic promoter variants revealed higher 2G allele basal and CSE-responsive activities than the 1G allele. Cotransfection, mithramycin, and electrophoretic mobility shift assay studies identified activating and repressive roles for Sp1 and PEA3 transcription factors, respectively. Oligonucleotide DNA arrays confirmed activation of Sp1 and PEA3 by CSE. These data demonstrate that the MMP-1 promoter is a direct target of cigarette smoke in lung epithelial cells. This characterization of a smoke response region in the distal MMP-1 promoter has implications for smoking-related diseases such as cancer, heart disease, and emphysema.
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