ArticleThe Journal of biological chemistry2008
Carbon dioxide transport through membranes.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed.
- Characterization and Computational Engineering of Structural Elements Controlling Gas Permeability in PIP2;1 Aquaporins.Journal of computational chemistry · 2026Article
- Simulating Gas Permeation Through the Central Pore of AQP5.Advances in experimental medicine and biology · 2026Article
- A pyrenoid-based CO2-concentrating mechanism can be effective and efficient even under scenarios of high membrane CO2 permeability.Plant physiology · 2025Article
- Article
- Modeling with uncertainty quantification reveals the essentials of a non-canonical algal carbon-concentrating mechanism.Plant physiology · 2025Article
- Reaction-diffusion modeling provides insights into biophysical carbon-concentrating mechanisms in land plants.Plant physiology · 2024Article
- Biological ammonium transporters from the Amt/Mep/Rh superfamily: mechanism, energetics, and technical limitations.Bioscience reports · 2024Review
- How is Membrane Permeation of Small Ionizable Molecules Affected by Protonation Kinetics?The journal of physical chemistry. B · 2024Article
- Biophysical carbon concentrating mechanisms in land plants: insights from reaction-diffusion modeling.bioRxiv : the preprint server for biology · 2024Article
- The role of chloroplast movement in C4 photosynthesis: a theoretical analysis using a three-dimensional reaction-diffusion model for maize.Journal of experimental botany · 2023Article
- Prokaryotic ammonium transporters: what has three decades of research revealed?Microbiology (Reading, England) · 2023Article
- Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.ACS synthetic biology · 2023Review
- OsαCA1 Affects Photosynthesis, Yield Potential, and Water Use Efficiency in Rice.International journal of molecular sciences · 2023Article
- Aquaporins and COFrontiers in physiology · 2023Review
- Controls on the isotopic composition of microbial methane.Science advances · 2022Article
- Biophysical Reviews' "Meet the Councilor Series"-a profile of Peter Pohl.Biophysical reviews · 2021Article
- Carbon dioxide transport across membranes.Interface focus · 2021Review
- Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in IsolatedFrontiers in plant science · 2021Article
- The control of acidity in tumor cells: a biophysical model.Scientific reports · 2020Article
- Sensing of tubular flow and renal electrolyte transport.Nature reviews. Nephrology · 2020Review
18 more citing papers are in PubMed but not listed here.
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7 authors.
Funding
Abstract
Several membrane channels, like aquaporin-1 (AQP1) and the RhAG protein of the rhesus complex, were hypothesized to be of physiological relevance for CO(2) transport. However, the underlying assumption that the lipid matrix imposes a significant barrier to CO(2) diffusion was never confirmed experimentally. Here we have monitored transmembrane CO(2) flux (J(CO2)) by imposing a CO(2) concentration gradient across planar lipid bilayers and detecting the resulting small pH shift in the immediate membrane vicinity. An analytical model, which accounts for the presence of both carbonic anhydrase and buffer molecules, was fitted to the experimental pH profiles using inverse problems techniques. At pH 7.4, the model revealed that J(CO2) was entirely rate-limited by near-membrane unstirred layers (USL), which act as diffusional barriers in series with the membrane. Membrane tightening by sphingomyelin and cholesterol did not alter J(CO2) confirming that membrane resistance was comparatively small. In contrast, a pH-induced shift of the CO(2) hydration-dehydration equilibrium resulted in a relative membrane contribution of about 15% to the total resistance (pH 9.6). Under these conditions, a membrane CO(2) permeability (3.2 +/- 1.6 cm/s) was estimated. It indicates that cellular CO(2) uptake (pH 7.4) is always USL-limited, because the USL size always exceeds 1 mum. Consequently, facilitation of CO(2) transport by AQP1, RhAG, or any other protein is highly unlikely. The conclusion was confirmed by the observation that CO(2) permeability of epithelial cell monolayers was always the same whether AQP1 was overexpressed in both the apical and basolateral membranes or not.
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