ArticleBiochemical and biophysical research communications2008
Proteomic analysis uncovers a metabolic phenotype in C. elegans after nhr-40 reduction of function.
Article in Biochemical and biophysical research communications, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Caenorhabditis elegans metabolic gene regulatory networks govern the cellular economy.Trends in endocrinology and metabolism: TEM · 2014Review
- RNA targets and specificity of Staufen, a double-stranded RNA-binding protein in Caenorhabditis elegans.The Journal of biological chemistry · 2013Article
- Diversification of fasting regulated transcription in a cluster of duplicated nuclear hormone receptors in C. elegans.Gene expression patterns : GEP · 2010Article
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Authors and funding
8 authors.
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Abstract
Caenorhabditis elegans has an unexpectedly large number (284) of genes encoding nuclear hormone receptors, most of which are nematode-specific and are of unknown function. We have exploited comparative two-dimensional chromatography of synchronized cultures of wild type C. elegans larvae and a mutant in nhr-40 to determine if proteomic approaches will provide additional insight into gene function. Chromatofocusing, followed by reversed-phase chromatography and mass spectrometry, identified altered chromatographic patterns for a set of proteins, many of which function in muscle and metabolism. Prompted by the proteomic analysis, we find that the penetrance of the developmental phenotypes in the mutant is enhanced at low temperatures and by food restriction. The combination of our phenotypic and proteomic analysis strongly suggests that NHR-40 provides a link between metabolism and muscle development. Our results highlight the utility of comparative two-dimensional chromatography to provide a relatively rapid method to gain insight into gene function.
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