ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2008
Development of an automated analysis system for data from flow cytometric intracellular cytokine staining assays from clinical vaccine trials.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- DNA and modified vaccinia virus Ankara vaccines encoding multiple cytotoxic and helper T-lymphocyte epitopes of human immunodeficiency virus type 1 (HIV-1) are safe but weakly immunogenic in HIV-1-uninfected, vaccinia virus-naive adults.Clinical and vaccine immunology : CVI · 2012Trial
- Timing of plasmid cytokine (IL-2/Ig) administration affects HIV-1 vaccine immunogenicity in HIV-seronegative subjects.The Journal of infectious diseases · 2011Trial
- HIV-DNA priming alters T cell responses to HIV-adenovirus vaccine even when responses to DNA are undetectable.Journal of immunology (Baltimore, Md. : 1950) · 2011Trial
- Trial
- Eight practices for data management to enable team data science.Journal of clinical and translational science · 2020Article
- High-throughput flow cytometry data normalization for clinical trials.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2014Article
- Quality control, analysis and secure sharing of Luminex® immunoassay data using the open source LabKey Server platform.BMC bioinformatics · 2013Article
- Automatic B cell lymphoma detection using flow cytometry data.BMC genomics · 2013Article
- QUAliFiER: an automated pipeline for quality assessment of gated flow cytometry data.BMC bioinformatics · 2012Article
- Computational analysis of high-throughput flow cytometry data.Expert opinion on drug discovery · 2012Review
- The average IFN-γ secreting capacity of specific CD8(+) T cells is compromised while increasing copies of a single T cell epitope encoded by DNA vaccine.Clinical & developmental immunology · 2012Article
- LabKey Server: an open source platform for scientific data integration, analysis and collaboration.BMC bioinformatics · 2011Article
- Correlation analysis of intracellular and secreted cytokines via the generalized integrated mean fluorescence intensity.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
- Data reduction for spectral clustering to analyze high throughput flow cytometry data.BMC bioinformatics · 2010Article
- HIV-1 vaccine-induced immunity in the test-of-concept Step Study: a case-cohort analysis.Lancet (London, England) · 2008Article
Corrections and comments
- Commented on by
Authors and funding
9 authors.
Funding
Abstract
Intracellular cytokine staining (ICS) by multiparameter flow cytometry is one of the primary methods for determining T-cell immunogenicity in HIV-1 clinical vaccine trials. Data analysis requires considerable expertise and time. The amount of data is quickly increasing as more and larger trials are performed, and thus there is a critical need for high-throughput methods of data analysis. A web-based flow cytometric analysis system, LabKey Flow, was developed for the analyses of data from standardized ICS assays. Using a gating template created manually in commercially available flow cytometric analysis software, the system automatically compensates and analyzes all data sets. Quality control queries were designed to identify potentially incorrect sample collections. Comparison of the semiautomated analysis performed by LabKey Flow and the manual analysis performed using FlowJo software demonstrated excellent concordance (concordance correlation coefficient > 0.990). Manual inspection of the analyses performed by LabKey Flow for eight-color ICS data files from several clinical vaccine trials indicated that template gates can appropriately be used for most data sets. Thus, the semiautomated LabKey Flow analysis system can accurately analyze large ICS data files. Routine use of the system does not require specialized expertise. This high-throughput analysis will provide great utility for rapid evaluation of complex multiparameter flow cytometric measurements collected from large clinical trials.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.