ArticlePlant physiology2008
Mobilization of rubisco and stroma-localized fluorescent proteins of chloroplasts to the vacuole by an ATG gene-dependent autophagic process.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 168 papers, 2 of them syntheses that pooled it.
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Who cites it
168 citing papers in PubMed, 2 syntheses or guidelines pooled it, 347 citations in OpenAlex.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).Autophagy · 2016Guideline
- Chloroplast Protein Degradation Pathways During Leaf Senescence: Coordination and Roles in Nitrogen Remobilization.Plants (Basel, Switzerland) · 2026Review
- Chloroplast stress caused by maltose hyperaccumulation activates chlorophagy via the core autophagy machinery.Plant physiology · 2026Article
- Selective degradation processes in plants are not limited to autophagy.Plant cell reports · 2026Review
- PLETHORA-autophagy axis activates organ regeneration through ROS modulation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Autophagy in plants: molecular mechanisms and roles in abiotic stress responses.Frontiers in plant science · 2026Review
- Review
- APE1 localizes to chloroplast stromules and interacts with ATI1 in Arabidopsis.Plant signaling & behavior · 2025Article
- Live-Cell Monitoring of Piecemeal Chloroplast Autophagy.Bio-protocol · 2025Article
- Autophagy plays a dual role in chromoplast transition and degradation and is essential for fruit coloration and ripening.Autophagy · 2025Article
- Cytoplasmic inheritance: The transmission of plastid and mitochondrial genomes across cells and generations.Plant physiology · 2025Review
- Article
- Vacuolar degradation of plant organelles.The Plant cell · 2024Review
- Intra-chloroplast proteases: A holistic network view of chloroplast proteolysis.The Plant cell · 2024Review
- Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants.The Plant cell · 2024Review
- Article
- Plastid Transient and Stable Interactions with Other Cell Compartments.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Studying plant autophagy: challenges and recommended methodologies.Advanced biotechnology · 2023Review
- Selective autophagy regulates chloroplast protein import and promotes plant stress tolerance.The EMBO journal · 2023Article
108 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During senescence and at times of stress, plants can mobilize needed nitrogen from chloroplasts in leaves to other organs. Much of the total leaf nitrogen is allocated to the most abundant plant protein, Rubisco. While bulk degradation of the cytosol and organelles in plants occurs by autophagy, the role of autophagy in the degradation of chloroplast proteins is still unclear. We have visualized the fate of Rubisco, stroma-targeted green fluorescent protein (GFP) and DsRed, and GFP-labeled Rubisco in order to investigate the involvement of autophagy in the mobilization of stromal proteins to the vacuole. Using immunoelectron microscopy, we previously demonstrated that Rubisco is released from the chloroplast into Rubisco-containing bodies (RCBs) in naturally senescent leaves. When leaves of transgenic Arabidopsis (Arabidopsis thaliana) plants expressing stroma-targeted fluorescent proteins were incubated with concanamycin A to inhibit vacuolar H(+)-ATPase activity, spherical bodies exhibiting GFP or DsRed fluorescence without chlorophyll fluorescence were observed in the vacuolar lumen. Double-labeled immunoelectron microscopy with anti-Rubisco and anti-GFP antibodies confirmed that the fluorescent bodies correspond to RCBs. RCBs could also be visualized using GFP-labeled Rubisco directly. RCBs were not observed in leaves of a T-DNA insertion mutant in ATG5, one of the essential genes for autophagy. Stroma-targeted DsRed and GFP-ATG8 fusion proteins were observed together in autophagic bodies in the vacuole. We conclude that Rubisco and stroma-targeted fluorescent proteins can be mobilized to the vacuole through an ATG gene-dependent autophagic process without prior chloroplast destruction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.