ArticleJournal of clinical microbiology2008
Comparison of conventional, nested, and real-time PCR assays for rapid and accurate detection of Vibrio vulnificus.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Validation of stool and blood analysis compared to Inv A and ttr based direct blood qPCR assay as diagnostic tools for typhoid fever.BMC microbiology · 2025Article
- Early diagnosis and application of hemopurification combined with antibiotic therapy and surgical debridement for successful treatment of a child with Vibrio vulnificus necrotizing fasciitis and septic shock: a case report.BMC infectious diseases · 2025Article
- Establishment of a 23S rRNA assay for Brucella and its application in evaluating bacterial growth status.Veterinary research communications · 2025Article
- Comparison of real-time PCR and nested PCR based on the HlyA gene for the detection of Listeria monocytogenes. Application on cheese samples.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Molecular epidemiology ofVeterinary world · 2023Article
- Successful surgical treatment of polybacterial gas gangrene confirmed by metagenomic next-generation sequencing detection: A case report.World journal of clinical cases · 2022Article
- Article
- Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample.Scientific reports · 2021Article
- High prevalence of Babesia microti in small mammals in Beijing.Infectious diseases of poverty · 2020Article
- Article
- An appraisal of oriental theileriosis and the Theileria orientalis complex, with an emphasis on diagnosis and genetic characterisation.Parasitology research · 2020Review
- A Retrospective Study about the Impact of Switching from Nested PCR to Multiplex Real-Time PCR on the Distribution of the Human Papillomavirus (HPV) Genotypes.Medicina (Kaunas, Lithuania) · 2019Article
- Vibrio vulnificus infection: a persistent threat to public health.The Korean journal of internal medicine · 2018Review
- Clinical Usefulness of Real-Time Polymerase Chain Reaction for the Diagnosis ofThe American journal of tropical medicine and hygiene · 2017Article
- Magnetic Nanoparticles PCR Enzyme-Linked Gene Assay for Quantitative Detection of BCR/ABL Fusion Gene in Chronic Myelogenous Leukemia.Journal of clinical laboratory analysis · 2016Article
- Evaluation of fliC-d based direct blood PCR assays for typhoid diagnosis.BMC microbiology · 2016Article
- Genome Wide Analysis for Rapid Identification of Vibrio Species.Indian journal of microbiology · 2015Article
- Quantitative PCR and in vivo efficacy of antibiotics in the treatment of Vibrio vulnificus infection in a mouse model.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Tumor necrosis factor-α and mortality in patients infected with Vibrio vulnificus.The American journal of tropical medicine and hygiene · 2011Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We conducted a prospective study to target toxR in the blood of patients with skin and soft tissue infections who were admitted to four tertiary hospitals to assess the clinical usefulness of real-time quantitative PCR (Q-PCR) as a diagnostic technique. We performed conventional PCR (C-PCR), nested PCR (N-PCR), and Q-PCR assays and compared the results to those obtained using the "gold standard" of microbiological culture. The lower detection limit for the Q-PCR assay was 5 x 10(0) copies/microl. By use of blood samples of patients with skin and soft tissue infections, the sensitivities of the C-PCR and N-PCR assays against the target toxR gene of V. vulnificus as diagnostic tools were determined to be 45% and 86%, respectively. The C-PCR and N-PCR assays had specificities of 100% and 73%, respectively. When we adopted a crossing-point (cp) cutoff value of <38 cp as a positive result, the Q-PCR assay had 100% sensitivity and specificity. Q-PCR to detect V. vulnificus-specific genes is not only the most sensitive and specific of the techniques but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay using blood is useful for the rapid diagnosis and subsequent treatment of V. vulnificus sepsis.
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