ArticleJournal of virology2008
Presence of the adenovirus IVa2 protein at a single vertex of the mature virion.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed.
- Adenovirus maturation establishes the transcription competent packaging of its genome.EMBO reports · 2025Article
- Chromatin structure and gene transcription of recombinant p53 adenovirus vector within host.Frontiers in molecular biosciences · 2025Article
- Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication.Science advances · 2024Article
- Review
- Adenovirus Core Proteins: Structure and Function.Viruses · 2021Review
- Recent Advances in Novel Antiviral Therapies against Human Adenovirus.Microorganisms · 2020Review
- Viral and cellular interactions during adenovirus DNA replication.FEBS letters · 2019Review
- Atomic Structures of Minor Proteins VI and VII in Human Adenovirus.Journal of virology · 2017Article
- Adenoviral E4 34K protein interacts with virus packaging components and may serve as the putative portal.Scientific reports · 2017Article
- The adenovirus major core protein VII is dispensable for virion assembly but is essential for lytic infection.PLoS pathogens · 2017Article
- Article
- Review
- Development of Potent Antiviral Drugs Inspired by Viral Hexameric DNA-Packaging Motors with Revolving Mechanism.Journal of virology · 2016Review
- Components of Adenovirus Genome Packaging.Frontiers in microbiology · 2016Review
- Structures of Adenovirus Incomplete Particles Clarify Capsid Architecture and Show Maturation Changes of Packaging Protein L1 52/55k.Journal of virology · 2015Article
- Adenoviral L4 33K forms ring-like oligomers and stimulates ATPase activity of IVa2: implications in viral genome packaging.Frontiers in microbiology · 2015Article
- Review
- Adenovirus composition, proteolysis, and disassembly studied by in-depth qualitative and quantitative proteomics.The Journal of biological chemistry · 2014Article
- Processing of the l1 52/55k protein by the adenovirus protease: a new substrate and new insights into virion maturation.Journal of virology · 2014Article
- The role of chromatin in adenoviral vector function.Viruses · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Assembly of adenovirus particles is thought to be similar to that of bacteriophages, in which the double-stranded DNA genome is inserted into a preformed empty capsid. Previous studies from our and other laboratories have implicated the viral IVa2 protein as a key component of the encapsidation process. IVa2 binds to the packaging sequence on the viral chromosome in a sequence-specific manner, alone and in conjunction with the viral L4 22K protein. In addition, it interacts with the viral L1 52/55-kDa protein, which is required for DNA packaging. Finally, a mutant virus that does not produce IVa2 is unable to produce any capsids. Therefore, it has been proposed that IVa2 nucleates capsid assembly. A prediction of such a model is that the IVa2 protein would be found at a unique vertex of the mature virion. In this study, the location of IVa2 in the virion has been analyzed using immunogold staining and electron microscopy, and the copy number of IVa2 in virions was determined using three independent methods, quantitative mass spectrometry, metabolic labeling, and Western blotting. The results indicate that it resides at a unique vertex and that there are approximately six to eight IVa2 molecules in each particle. These findings support the hypothesis that the IVa2 protein plays multiple roles in the viral assembly process.
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Registered trials
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