ArticleThe Journal of biological chemistry2008
A block in endoplasmic reticulum-to-Golgi trafficking inhibits phospholipid synthesis and induces neutral lipid accumulation.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Rescue of Hepatic Phospholipid Remodeling Defectin iPLA2β-Null Mice Attenuates Obese but Not Non-Obese Fatty Liver.Biomolecules · 2020Review
- Interaction between repressor Opi1p and ER membrane protein Scs2p facilitates transit of phosphatidic acid from the ER to mitochondria and is essential forThe Journal of biological chemistry · 2017Article
- The protein and neutral lipid composition of lipid droplets isolated from the fission yeast, Schizosaccharomyces pombe.Journal of microbiology (Seoul, Korea) · 2017Article
- Lipid partitioning at the nuclear envelope controls membrane biogenesis.Molecular biology of the cell · 2015Article
- Regulation of lipid droplet dynamics in Saccharomyces cerevisiae depends on the Rab7-like Ypt7p, HOPS complex and V1-ATPase.Biology open · 2015Article
- Article
- The lipid droplet-a well-connected organelle.Frontiers in cell and developmental biology · 2015Review
- Arabidopsis lipins, PDAT1 acyltransferase, and SDP1 triacylglycerol lipase synergistically direct fatty acids toward β-oxidation, thereby maintaining membrane lipid homeostasis.The Plant cell · 2014Article
- The response to inositol: regulation of glycerolipid metabolism and stress response signaling in yeast.Chemistry and physics of lipids · 2014Review
- The emergence of lipid droplets in yeast: current status and experimental approaches.Current genetics · 2013Review
- Triacylglycerol mobilization is suppressed by brefeldin A in Chlamydomonas reinhardtii.Plant & cell physiology · 2013Article
- Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down.Genetics · 2013Review
- Identification of diverse lipid droplet targeting motifs in the PNPLA family of triglyceride lipases.PloS one · 2013Article
- Rapid induction of lipid droplets in Chlamydomonas reinhardtii and Chlorella vulgaris by Brefeldin A.PloS one · 2013Article
- SCS3 and YFT2 link transcription of phospholipid biosynthetic genes to ER stress and the UPR.PLoS genetics · 2012Article
- Controlling lipid fluxes at glycerol-3-phosphate acyltransferase step in yeast: unique contribution of Gat1p to oleic acid-induced lipid particle formation.The Journal of biological chemistry · 2012Article
- Review
- The yeast magmas ortholog pam16 has an essential function in fermentative growth that involves sphingolipid metabolism.PloS one · 2012Article
- Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy.PloS one · 2012Article
- White lupin cluster root acclimation to phosphorus deficiency and root hair development involve unique glycerophosphodiester phosphodiesterases.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Seeking to better understand how membrane trafficking is coordinated with phospholipid synthesis in yeast, we investigated lipid synthesis in several Sec(-) temperature-sensitive mutants, including sec13-1. Upon shift of sec13-1 cells to the restrictive temperature of 37 degrees C, phospholipid synthesis decreased dramatically relative to the wild type control, whereas synthesis of neutral lipids, especially triacylglycerol (TAG), increased. When examined by fluorescence microscopy, the number of lipid droplets appeared to increase and formed aggregates in sec13-1 cells shifted to 37 degrees C. Electron microscopy confirmed the increase in lipid droplet number and revealed that many were associated with the vacuole. Analysis of lipid metabolism in strains lacking TAG synthase genes demonstrated that the activities of the products of these genes contribute to accumulation of TAG in sec13-1 cells after the shift to 37 degrees C. Furthermore, the permissive temperature for growth of the sec13-1 strain lacking TAG synthase genes was 3 degrees C lower than sec13-1 on several different growth media, indicating that the synthesis of TAG has physiological significance under conditions of secretory stress. Together these results suggest that following a block in membrane trafficking, yeast cells channel lipid metabolism from phospholipid synthesis into synthesis of TAG and other neutral lipids to form lipid droplets. We conclude that this metabolic switch provides a degree of protection to cells during secretory stress.
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Registered trials
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