ArticlePLoS biology2008
A short-time scale colloidal system reveals early bacterial adhesion dynamics.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Characterization of functional amyloid curli in biofilm formation of an environmental isolate Enterobacter cloacae SBP-8.Antonie van Leeuwenhoek · 2023Article
- Influence of Surface Roughness, Nanostructure, and Wetting on Bacterial Adhesion.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Natural Strategies as Potential Weapons against Bacterial Biofilms.Life (Basel, Switzerland) · 2022Review
- Shiga Toxin-ProducingMicroorganisms · 2022Review
- Flagellar Motility DuringFrontiers in cellular and infection microbiology · 2022Article
- Cold plasma surface treatments to prevent biofilm formation in food industries and medical sectors.Applied microbiology and biotechnology · 2022Review
- How bacteria recognise and respond to surface contact.FEMS microbiology reviews · 2020Review
- The influence of surface chemistry on the kinetics and thermodynamics of bacterial adhesion.Scientific reports · 2018Article
- Fouling-Resistant Hydrogels Prepared by the Swelling-Assisted Infusion and Polymerization of Dopamine.ACS applied bio materials · 2018Article
- Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion.Science advances · 2018Article
- Asymmetric adhesion of rod-shaped bacteria controls microcolony morphogenesis.Nature communications · 2018Article
- Bacterial autoaggregation.AIMS microbiology · 2018Review
- Adhesion mechanisms of curli subunit CsgA to abiotic surfaces.Science advances · 2016Article
- Multi-disciplinary antimicrobial strategies for improving orthopaedic implants to prevent prosthetic joint infections in hip and knee.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Review
- Fewer Bacteria Adhere to Softer Hydrogels.ACS applied materials & interfaces · 2015Article
- Surfaces Presenting α-Phenyl Mannoside Derivatives Enable Formation of Stable, High Coverage, Non-pathogenicBiomaterials science · 2015Article
- Bacteria repelling on highly-ordered alumina-nanopore structures.Journal of applied physics · 2015Article
- Article
- Impact of Lactococcus lactis spp. lactis Bio Adhesion on Pathogenic Bacillus cereus Biofilm on Silicone Flowing System.Indian journal of microbiology · 2013Article
- An electrically reversible switchable surface to control and study early bacterial adhesion dynamics in real-time.Advanced materials (Deerfield Beach, Fla.) · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of bacteria on abiotic surfaces has important public health and sanitary consequences. However, despite several decades of study of bacterial adhesion to inert surfaces, the biophysical mechanisms governing this process remain poorly understood, due, in particular, to the lack of methodologies covering the appropriate time scale. Using micrometric colloidal surface particles and flow cytometry analysis, we developed a rapid multiparametric approach to studying early events in adhesion of the bacterium Escherichia coli. This approach simultaneously describes the kinetics and amplitude of early steps in adhesion, changes in physicochemical surface properties within the first few seconds of adhesion, and the self-association state of attached and free-floating cells. Examination of the role of three well-characterized E. coli surface adhesion factors upon attachment to colloidal surfaces--curli fimbriae, F-conjugative pilus, and Ag43 adhesin--showed clear-cut differences in the very initial phases of surface colonization for cell-bearing surface structures, all known to promote biofilm development. Our multiparametric analysis revealed a correlation in the adhesion phase with cell-to-cell aggregation properties and demonstrated that this phenomenon amplified surface colonization once initial cell-surface attachment was achieved. Monitoring of real-time physico-chemical particle surface properties showed that surface-active molecules of bacterial origin quickly modified surface properties, providing new insight into the intricate relations connecting abiotic surface physicochemical properties and bacterial adhesion. Hence, the biophysical analytical method described here provides a new and relevant approach to quantitatively and kinetically investigating bacterial adhesion and biofilm development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.