ReviewCytometry. Part A : the journal of the International Society for Analytical Cytology2008
Antigen-specific B cell detection reagents: use and quality control.
Review in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Single B-cell deconvolution of peanut-specific antibody responses in allergic patients.The Journal of allergy and clinical immunology · 2016Trial
- Humoral and Cell-Mediated Immunity Against SARS-CoV-2 in Healthcare Personnel Who Received Multiple mRNA Vaccines: A 4-Year Observational Study.Infectious disease reports · 2025Article
- Single-residue linear and conformational B cell epitopes prediction using random and ESM-2 based projections.Briefings in bioinformatics · 2024Article
- Quality-controlled characterization of a monoclonal antibody specific to an EC5-domain of human desmoglein 3 for pemphigus research.Frontiers in immunology · 2024Article
- Validation of Ligand Tetramers for the Detection of Antigen-Specific Lymphocytes.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Simultaneous analysis of antigen-specific B and T cells after SARS-CoV-2 infection and vaccination.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Article
- Efficient isolation of rare B cells using next-generation antigen barcoding.Frontiers in cellular and infection microbiology · 2022Article
- Tetrameric Fluorescent Antigen Arrays for Single-Step Identification of Antigen-Specific B Cells.Journal of visualized experiments : JoVE · 2020Article
- Single-Cell Analysis of CD4 T Cells in Type 1 Diabetes: From Mouse to Man, How to Perform Mechanistic Studies.Diabetes · 2019Article
- Approaches to Interrogating the Human Memory B-Cell and Memory-Derived Antibody Repertoire Following Dengue Virus Infection.Frontiers in immunology · 2019Review
- Techniques to Study Antigen-Specific B Cell Responses.Frontiers in immunology · 2019Review
- Detection, phenotyping and quantification of dengue virus-specific B cells using fluorescent probes.Human vaccines & immunotherapeutics · 2017Article
- Optimisation ofWellcome open research · 2017Article
- Rapid profiling of RSV antibody repertoires from the memory B cells of naturally infected adult donors.Science immunology · 2016Article
- Fluorescently labeled dengue viruses as probes to identify antigen-specific memory B cells by multiparametric flow cytometry.Journal of immunological methods · 2015Article
- Article
- Optimized fluorescent labeling to identify memory B cells specific for Neisseria meningitidis serogroup B vaccine antigens ex vivo.Immunity, inflammation and disease · 2013Article
- Immunopathogenesis of simian immunodeficiency virus infection in nonhuman primates.Current opinion in HIV and AIDS · 2013Review
- Memory B cells from a subset of treatment-naïve relapsing-remitting multiple sclerosis patients elicit CD4(+) T-cell proliferation and IFN-γ production in response to myelin basic protein and myelin oligodendrocyte glycoprotein.European journal of immunology · 2010Article
- Measuring antigen-specific immune responses, 2008 update.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Tests for immunoglobulin reactivity with specific antigens are some of the oldest and most used assays in immunology. With efforts to understand B cell development, B cell dysregulation in autoimmunity, and to generate B cell vaccines for infectious agents, investigators have found the need to understand the ontogeny and regulation of epitope-specific B cell responses. The synchrony between surface and secreted antibodies for individual B cells has led to the development of reagents and techniques to identify antigen-specific B cells via reagent interactions with the B cell receptor complex. B cell antigen-specific reagents have been reported for model systems of haptens, for whole proteins, and for identification of double stranded (ds) DNA antibody-producing B cells using peptide mimics. Here we provide an overview of reported techniques for the detection of antigen-specific B cell responses via secreted antibody or by the surface B cell receptor and briefly discuss our recent work developing a panel of reagents to probe the B cell response to HIV-1 envelope. We also present an analysis of strengths and weaknesses of various methods for flow cytometric analysis of antigen-specific B cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.