ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2008
Optimization of yeast cell cycle analysis and morphological characterization by multispectral imaging flow cytometry.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 65 citations in OpenAlex.
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- Dynamic characterization of growth and gene expression using high-throughput automated flow cytometry.Nature methods · 2014Article
- Control analysis of the eukaryotic cell cycle using gene copy-number series in yeast tetraploids.BMC genomics · 2013Article
- Automated quantification of budding Saccharomyces cerevisiae using a novel image cytometry method.Journal of industrial microbiology & biotechnology · 2013Article
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- Attachment and invasion of Neisseria meningitidis to host cells is related to surface hydrophobicity, bacterial cell size and capsule.PloS one · 2013Article
- Apn1 AP-endonuclease is essential for the repair of oxidatively damaged DNA bases in yeast frataxin-deficient cells.Human molecular genetics · 2012Article
- High-throughput single-microparticle imaging flow analyzer.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Life, death, and in-between: meanings and methods in microbiology.Applied and environmental microbiology · 2011Review
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3 authors at 1 institution in 1 country.
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Abstract
Budding yeast Saccharoymyces cerevisiae is a powerful model system for analyzing eukaryotic cell cycle regulation. Yeast cell cycle analysis is typically performed by visual analysis or flow cytometry, and both have limitations in the scope and accuracy of data obtained. This study demonstrates how multispectral imaging flow cytometry (MIFC) provides precise quantitation of cell cycle distribution and morphological phenotypes of yeast cells in flow. Cell cycle analysis of wild-type yeast, nap1Delta, and yeast overexpressing NAP1, was performed visually, by flow cytometry and by MIFC. Quantitative morphological analysis employed measurements of cellular length, thickness, and aspect ratio in an algorithm to calculate a novel feature, bud length. MIFC demonstrated reliable quantification of the yeast cell cycle compared to morphological and flow cytometric analyses. By employing this technique, we observed both the G2/M delay and elongated buds previously described in the nap1Delta strain. Using MIFC, we demonstrate that overexpression of NAP1 causes elongated buds yet only a minor disruption in the cell cycle. The different effects of NAP1 expression level on cell cycle and morphology suggests that these phenotypes are independent. Unlike conventional yeast flow cytometry, MIFC generates complete cell cycle profiles and concurrently offers multiple parameters for morphological analysis.
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