ArticleMolecular and cellular biochemistry2008
Ubiquitin over-expression promotes E6AP autodegradation and reactivation of the p53/MDM2 pathway in HeLa cells.
Article in Molecular and cellular biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Ubiquitin E3 Ligases and p53 in Doxorubicin-Induced Cardiotoxicity.International journal of molecular sciences · 2025Review
- Proteomic characterization and cytotoxic potential of proteins from Cuscuta (Cuscuta epithymum (L.) crude herbal product against MCF-7 human breast cancer cell line.BMC complementary medicine and therapies · 2024Article
- The Ubiquitin Gene Expression Pattern and Sensitivity toInternational journal of molecular sciences · 2020Article
- A negative feedback mechanism links UBC gene expression to ubiquitin levels by affecting RNA splicing rather than transcription.Scientific reports · 2019Article
- Induction ofFEBS open bio · 2018Article
- The Autism Protein Ube3A/E6AP Remodels Neuronal Dendritic Arborization via Caspase-Dependent Microtubule Destabilization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Efficacy of recombinant human adenovirus-p53 combined with chemotherapy for locally advanced cervical cancer: A clinical trial.Oncology letters · 2017Article
- Article
- Evaluation of selected binding domains for the analysis of ubiquitinated proteomes.Journal of the American Society for Mass Spectrometry · 2013Article
- Alix serves as an adaptor that allows human parainfluenza virus type 1 to interact with the host cell ESCRT system.PloS one · 2013Article
- Sequential posttranslational modifications program FEN1 degradation during cell-cycle progression.Molecular cell · 2012Article
- Non-covalent interaction between polyubiquitin and GTP cyclohydrolase 1 dictates its degradation.PloS one · 2012Article
- YY1 is a novel potential therapeutic target for the treatment of HPV infection-induced cervical cancer by arsenic trioxide.International journal of gynecological cancer : official journal of the International Gynecological Cancer Society · 2011Article
- Angelman syndrome: insights into genomic imprinting and neurodevelopmental phenotypes.Trends in neurosciences · 2011Review
- De-ubiquitylation is the most critical step in the ubiquitin-mediated homeostatic control of the NF-kappaB/IKK basal activity.Molecular and cellular biochemistry · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been established that intracellular ubiquitin pools are subject to regulatory constrains. Less certain is the mechanism by which the pool of conjugated ubiquitin shift in parallel with total ubiquitin, and how this type of regulation affects the flux of substrates through the pathway. In this study we demonstrate that ubiquitin over-expression promotes the destabilization of the ubiquitin protein ligase E6AP, by a mechanism involving self-ubiquitination, and the stabilization of p53. These results represent the very first evidence that the levels of a ubiquitin ligase can be regulated in vivo by ubiquitin abundance, supporting the idea that a strict interrelationship between pathway component activities and ubiquitin pool size exists. Interestingly, ubiquitin-induced p53 accumulation did not induce cell-cycle arrest, suggesting that although fluctuations of the intracellular ubiquitin content may actively modulate the level of regulatory proteins, this event is not per se sufficient to elicit a cellular response in terms of proliferation.
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