ArticleThe Journal of biological chemistry2008
Ribosome performance is enhanced by a rich cluster of pseudouridines in the A-site finger region of the large subunit.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 66 citations in OpenAlex.
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- Exploring the roles of snoRNA-induced ribosome heterogeneity in equine osteoarthritis.Frontiers in veterinary science · 2025Article
- Effects of Mitochondrial Transplantation on Transcriptomics in a Polymicrobial Sepsis Model.International journal of molecular sciences · 2023Article
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- CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations.Structure (London, England : 1993) · 2022Article
- The plant epitranscriptome: revisiting pseudouridine and 2'-O-methyl RNA modifications.Plant biotechnology journal · 2022Review
- Ribosomal RNA Pseudouridylation: Will Newly Available Methods Finally Define the Contribution of This Modification to Human Ribosome Plasticity?Frontiers in genetics · 2022Review
- Small but Mighty-The Emerging Role of snoRNAs in Hematological Malignancies.Non-coding RNA · 2021Review
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- snR30/U17 Small Nucleolar Ribonucleoprotein: A Critical Player during Ribosome Biogenesis.Cells · 2020Review
- H/ACA snoRNA levels are regulated during stem cell differentiation.Nucleic acids research · 2020Article
- Ribosomes: An Exciting Avenue in Stem Cell Research.Stem cells international · 2020Review
- SHAPE Probing Reveals Human rRNAs Are Largely Unfolded in Solution.Biochemistry · 2019Article
- The chemical diversity of RNA modifications.The Biochemical journal · 2019Review
- Post-transcriptional pseudouridylation in mRNA as well as in some major types of noncoding RNAs.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Review
- Contribution of protein Gar1 to the RNA-guided and RNA-independent rRNA:Ψ-synthase activities of the archaeal Cbf5 protein.Scientific reports · 2018Article
- RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.Chemical reviews · 2018Review
- Review
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The large subunit rRNA in eukaryotes contains an unusually dense cluster of 8-10 pseudouridine (Psi) modifications located in a three-helix structure (H37-H39) implicated in several functions. This region is dominated by a long flexible helix (H38) known as the "A-site finger" (ASF). The ASF protrudes from the large subunit just above the A-site of tRNA binding, interacts with 5 S rRNA and tRNA, and through the terminal loop, forms a bridge (B1a) with the small subunit. In yeast, the three-helix domain contains 10 Psis and 6 are concentrated in the ASF helix (3 of the ASF Psis are conserved among eukaryotes). Here, we show by genetic depletion analysis that the Psis in the ASF helix and adjoining helices are not crucial for cell viability; however, their presence notably enhances ribosome fitness. Depleting different combinations of Psis suggest that the modification pattern is important and revealed that loss of multiple Psis negatively influences ribosome performance. The effects observed include slower cell growth (reduced rates up to 23% at 30 degrees C and 40-50% at 37 degrees C and 11 degrees C), reduced level of the large subunit (up to 17%), impaired polysome formation (appearance of half-mers), reduced translation activity (up to 20% at 30 degrees C and 25% at 11 degrees C), and increased sensitivity to ribosome-based drugs. The results indicate that the Psis in the three-helix region improve fitness of a eukaryotic ribosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.