ArticleClinical chemistry and laboratory medicine2008
Comprehensive profiling of the human circulating endocannabinoid metabolome: clinical sampling and sample storage parameters.
Article in Clinical chemistry and laboratory medicine, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 72 citations in OpenAlex.
- Trial
- Acute stress increases circulating anandamide and other N-acylethanolamines in healthy humans.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2012Trial
- Palmitoylethanolamide in Human and Animal Obesity.Molecules (Basel, Switzerland) · 2026Review
- A Minimally Invasive LC-MS/MS Approach for Assessing Endocannabinoids in Saliva and Capillary Blood Microsamples.Biosensors · 2026Article
- Altered Plasma Endocannabinoids and Oxylipins in Adolescents with Major Depressive Disorders: A Case-Control Study.Nutrients · 2026Article
- Genetic variation of the 5-HT1A rs6295, 5-HT2A rs6311, and CNR1 rs1049353 and an altered endocannabinoid system in depressed patients.Brain and behavior · 2023Article
- Article
- The endocannabinoid system in humans: significant associations between anandamide, brain function during reward feedback and a personality measure of reward dependence.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2021Article
- Stability of amino acids and related amines in human serum under different preprocessing and pre-storage conditions based on iTRAQBiology open · 2021Article
- Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics.Metabolites · 2020Review
- Sphingolipidomics in Translational Sepsis Research-Biomedical Considerations and Perspectives.Frontiers in medicine · 2020Review
- Pre-Analytical Factors that Affect Metabolite Stability in Human Urine, Plasma, and Serum: A Review.Metabolites · 2019Review
- Cerebral Vitamin B5 (D-Pantothenic Acid) Deficiency as a Potential Cause of Metabolic Perturbation and Neurodegeneration in Huntington's Disease.Metabolites · 2019Article
- Endocannabinoid Metabolome Characterization of Milk from Guatemalan Women Living in the Western Highlands.Current developments in nutrition · 2019Article
- Profiling plasma N-Acylethanolamine levels and their ratios as a biomarker of obesity and dysmetabolism.Molecular metabolism · 2018Article
- Determination of the Endocannabinoids Anandamide and 2-Arachidonoyl Glycerol with Gas Chromatography-Mass Spectrometry: Analytical and Preanalytical Challenges and Pitfalls.Medical cannabis and cannabinoids · 2018Article
- Plasma 2-arachidonoylglycerol is a biomarker of age and menopause related insulin resistance and dyslipidemia in lean but not in obese men and women.Molecular metabolism · 2017Article
- Shotgun Lipidomics Approach to Stabilize the Regiospecificity of Monoglycerides Using a Facile Low-Temperature Derivatization Enabling Their Definitive Identification and Quantitation.Analytical chemistry · 2016Article
- Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification.Neural plasticity · 2016Review
- Diet-induced changes in n-3- and n-6-derived endocannabinoids and reductions in headache pain and psychological distress.The journal of pain · 2015Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundEndogenous cannabinoid-receptor ligands (endocannabinoids) and over a dozen related metabolites now comprise the "endocannabinoid metabolome". The diverse (patho)physiological roles of endocannabinoids, the predictive/diagnostic utility of systemic endocannabinoid levels, and the growing interest in endocannabinoid-related pharmacotherapeutics mandate a valid clinical protocol for processing human blood that does not jeopardize profiling of the circulating endocannabinoid metabolome.
methodsWe systematically evaluated the potential effect of pre-analytical variables associated with phlebotomy and sample handling/work-up on the human-blood endocannabinoid metabolome as quantified by state-of-the-art liquid chromatography-mass spectrometry.
resultsNeither subject posture during phlebotomy nor moderate activity beforehand influenced the blood levels of the 15 endocannabinoid-system lipids quantified. Storage of fresh blood at 4 degrees C selectively enhanced ethanolamide concentrations artifactually without affecting monoglycerides and nonesterified fatty acids, such as arachidonic acid. In marked contrast, ethanolamides and monoglycerides remained stable through three plasma freeze/thaw cycles, whereas plasma arachidonic acid content increased, probably a reflection of ongoing metabolism.
conclusionsClass- and compound-selective pre-analytical influences on circulating human endocannabinoid levels necessitate immediate plasma preparation from fresh blood and prompt plasma apportioning and snap-freezing. Repeated plasma thawing and refreezing should be avoided. This protocol ensures sample integrity for evaluating the circulating endocannabinoid metabolome in the clinical setting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.