ArticleWorld journal of gastroenterology2008
PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer.
Article in World journal of gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Pathogenetic mechanisms in gastric cancer.World journal of gastroenterology · 2014Review
- Promoter region methylation and loss of protein expression of PTEN and significance in cervical cancer.Biomedical reports · 2014Article
- [Research progress of the relationship between tumor suppressor gene PTEN and non-small cell lung cancer].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2014Review
- Occipital anaplastic oligodendroglioma with multiple organ metastases after a short clinical course: a case report and literature review.Diagnostic pathology · 2014Review
- Gastric cancer-molecular and clinical dimensions.Nature reviews. Clinical oncology · 2013Review
- Immunohistochemical expression of mTOR negatively correlates with PTEN expression in gastric carcinoma.Oncology letters · 2012Article
- Aberrant promoter methylation and inactivation of PTEN gene in cervical carcinoma from Indian population.Journal of cancer research and clinical oncology · 2011Article
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo discuss the possible effect of PTEN gene mutations on occurrence and development of gastric cancer.
methodsFifty-three gastric cancer specimens were selected to probe PTEN gene mutations in genome of gastric cancer and paracancerous tissues using PCR-SSCP-DNA sequencing method based on microdissection and to observe the protein expression by immunohistochemistry technique.
resultsPCR-SSCP-DNA sequencing indicated that 4 kinds of mutation sites were found in 5 of 53 gastric cancer specimens. One kind of mutation was found in exons. AA-TCC mutation was located at 40 bp upstream of 3' lateral exon 7 (115946 AA-TCC). Such mutations led to terminator formation in the 297th codon of the PTEN gene. The other 3 kinds of mutation were found in introns, including a G-C point mutation at 91 bp upstream of 5' lateral exon 5(90896 G-C), a T-G point mutation at 24 bp upstream of 5' lateral exon 5 (90963 T-G), and a single base A mutation at 7 bp upstream of 5' lateral exon 5 (90980 A del). The PTEN protein expression in gastric cancer and paracancerous tissues detected using immunohistochemistry technique indicated that the total positive rate of PTEN protein expression was 66% in gastric cancer tissue, which was significantly lower than that (100%) in paracancerous tissues (P < 0.005).
conclusionPTEN gene mutation and expression may play an important role in the occurrence and development of gastric cancer.
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