ArticleThe Journal of cell biology1991
Characterization of a new human osteosarcoma cell line OHS-4.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 48 citations in OpenAlex.
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- Acquisition of cancer stem cell properties in osteosarcoma cells by defined factors.Stem cell research & therapy · 2020Article
- Cells Isolated from Regenerating Caudal Fin of Sparus aurata Can Differentiate into Distinct Bone Cell Lineages.Marine biotechnology (New York, N.Y.) · 2020Article
- MT2A is an early predictive biomarker of response to chemotherapy and a potential therapeutic target in osteosarcoma.Scientific reports · 2019Article
- Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization.Zebrafish · 2013Article
- Development and characterization of Xl1, a Xenopus laevis chondrocyte-like cell culture.Molecular and cellular biochemistry · 2013Article
- [In vitro trials with single and co-cultures of osteoblasts and endothelial cells : evaluation of new biomaterials for bone reconstruction and regeneration].Der Orthopade · 2009Article
- Establishment of a new human osteosarcoma cell line, UTOS-1: cytogenetic characterization by array comparative genomic hybridization.Journal of experimental & clinical cancer research : CR · 2009Article
- Establishment of primary cell cultures from fish calcified tissues.Cytotechnology · 2007Article
- Study on angiogenesis factor of human osteosarcoma.Journal of Tongji Medical University = Tong ji yi ke da xue xue bao · 2000Article
- Osteoblasts are target cells for transformation in c-fos transgenic mice.The Journal of cell biology · 1993Article
- Establishment and characterization of a simian virus 40-immortalized osteoblastic cell line from normal human bone.Japanese journal of cancer research : Gann · 1993Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a new human osteosarcoma cell line designated OHS-4. These cells showed a high alkaline phosphatase activity that is not regulated by 1,25 dihydroxyvitamin D3. They exhibited a sensitive adenylate cyclase response to parathyroid hormone but not to prostaglandin E2 or human calcitonin. By Northern blot analysis we could detect type I collagen mRNA but none for type III collagen. The cells were able to produce human osteocalcin at a maximum level of 35 ng per million cells when exposed to 2.4 nM 1,25-dihydroxyvitamin D3 for 96 h. We purified this protein from conditioned media using successive chromatography and assessed its identity by partial amino acid sequencing. When injected into nude mice, the cells retained their osteogenic activity and developed calcified tumors. After Von Kossa staining, we observed nonmineralized osteoid deposits and mineralized deposits with a structure similar to that of trabecular bone by light microscopy. On the basis of its osteoblastic characteristics, this new osteosarcoma cell line may represent the human counterpart of the ROS 17/2 cell line. This cell line represents a valuable model for the isolation and characterization of human bone specific proteins.
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