ArticleJournal of molecular neuroscience : MN2009
Selection of reference genes for quantitative real-time RT-PCR studies in mouse brain.
Article in Journal of molecular neuroscience : MN, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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79 citing papers in PubMed.
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- Reference gene variability across age and sex in 5XFAD mice highlights normalization challenges in Alzheimer's models.Scientific reports · 2026Article
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- Oxytocin treatment at birth accelerates an epigenetic shift in the oxytocin receptor gene in the maternal brain.BMC pregnancy and childbirth · 2025Article
- Nicorandil treatment improves survival and spatial learning in aged granulin knockout mice.Brain pathology (Zurich, Switzerland) · 2025Article
- Identification of an appropriate reference gene for normalization of qRT-PCR expression analyses in human breast cancer cell lines: application to L-arginine depletion studies.Journal of cancer research and clinical oncology · 2025Article
- Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR.BMC neuroscience · 2025Article
- Validation of RNA Extraction Methods and Suitable Reference Genes for Gene Expression Studies in Developing Fetal Human Inner Ear Tissue.International journal of molecular sciences · 2024Article
- Cross-species epigenetic regulation of nucleus accumbens KCNN3 transcripts by excessive ethanol drinking.Translational psychiatry · 2023Article
- Impact of thyroid hormone perturbations in adult mice: brain weight and blood vessel changes, gene expression variation, and neurobehavioral outcomes.Neurobiology of aging · 2023Article
- Cross-hierarchical plasticity of corticofugal projections to dLGN after neonatal monocular enucleation.The Journal of comparative neurology · 2022Article
- Targeted Suppression of Lipoprotein Receptor LSR in Astrocytes Leads to Olfactory and Memory Deficits in Mice.International journal of molecular sciences · 2022Article
- RNA-Seq is not required to determine stable reference genes for qPCR normalization.PLoS computational biology · 2022Article
- Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma.Frontiers in genetics · 2022Article
- An approach to quantitate maternal transcripts localized in sea urchin egg cortex using RT-qPCR with accurate normalization.PloS one · 2022Article
- Neurobeachin, a promising target for use in the treatment of alcohol use disorder.Addiction biology · 2022Article
- Genetic, epigenetic, and environmental factors controlling oxytocin receptor gene expression.Clinical epigenetics · 2021Article
- Identification and Validation of Reference Genes inFrontiers in microbiology · 2021Article
19 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
Since a growing number of studies based on the real-time reverse transcriptase polymerase chain reaction (RT-PCR) continue to be published in order to highlight genes specifically involved in brain development, maturation, and function, the identification of reference genes suitable for this kind of experiments is now an urgent need in the neuroscience field. The aim of this work was to verify the suitability of some very common housekeeping genes (such as Gapdh, 18s, and B2m) and of some relatively new control genes (such as Pgk1, Tfrc, and Gusb) during mouse brain maturation. We tested the candidate reference genes in mouse whole brain, cerebellum, brain stem, hippocampus, medial septum, frontal neocortex, and olfactory bulb. Moreover, we reported the first complete study of Pgk1 expression throughout the development and the aging of mouse brain. Although no tested gene showed to be the optimal reference for all mouse brain regions, in general, the new housekeeping genes were highly stable in most of the analyzed regions. Above all, with few exceptions, Pgk1 showed to be a reliable control for the analyzed mouse brain regions during development, maturation, and aging.
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